The protein pyruvate carboxylase is a catalyst for one step (of many) in the conversion of food molecules into ATP, a molecule that serves as a common energy carrier in the cell. In more detail, the enzyme assists the (reversible) conversion from pyruvate, bicarbonate, and ATP into oxaloacetate, ADP (sort of a reduced energy version of ATP), and phosphorus. There is a sequential set of enzyme-assisted chemical reactions which starts with a sugar molecule, glucose, and produces ATP. This set of steps commonly occurs in eukaryotes. The stage called glycolysis takes glucose and produces pyruvate, and other molecules. In eukaryotes, there is a bacteria known as mitochondria that lives in symbiosis with the cell. The mitochondria takes in pyruvate and pyruvate carboxylase in order to start a series of reactions called the citric acid cycle that eventually produces more ATP. In mammals, pyruvate carboxylase is typically expressed in greater amounts in the liver than in other tissues. In Bacillus subtilis the enzyme plays a similar role in processing glucose, however not all prokaryotes use pyruvate carboxylase [Sauer et. al, 1998; Jitrapakdee and Wallace, 1999].
Score = 2987 (1051.5 bits), Expect = 1.6e-312, P = 1.6e-312
Identities = 590/1147 (51%), Positives = 780/1147 (68%)
Query: 1 MSQQSIQKVLVANRGEIAIRIFRACTELNIRTVAVYSKEDSGSYHRYKADEAYLVGEGKK 60
+ + I+KV+VANRGEIAIR+FRACTEL IRTVA+YS++D+G HR KADEAYL+G G
Sbjct: 32 LEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLA 91
Query: 61 PIDAYLDIEGIIDIAKRNKVDAIHPGYGFLSENIHFARRCEEEGIVFIGPKSEHLDMFGD 120
P+ AYL I II +AK N VDA+HPGYGFLSE FA+ C++ G+ FIGP E + GD
Sbjct: 92 PVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGD 151
Query: 121 KVKAREQAEKAGIPVIPGSDGPAETLEAVEQFGQANGYPIIIKASLGGGGRGMRIVRSES 180
KV+AR A AG+PV+PG+D P +L +F G+PII KA+ GGGGRGMR+V S
Sbjct: 152 KVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYE 211
Query: 181 EVKEAYERAKSEAKAAFGNDEVYVEKLIENPKHIEVQVIGDKQGNVVHLFERDCSVQRRH 240
E++E Y RA SEA AAFGN ++VEK IE P+HIEVQ++GD+ GN++HL+ERDCS+QRRH
Sbjct: 212 ELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRH 271
Query: 241 QKVIEVAPSVSLSPELRDQICEAAVALAKNVNYINAGTVEFLV-ANNEFYFIEVNPRVQV 299
QKV+E+AP+ L P+LR ++ +V LAK V Y NAGTVEFLV + + YFIEVN R+QV
Sbjct: 272 QKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQV 331
Query: 300 EHTITEMITGVDIVQTQILVAQGHSLHSKKVNIPEQKDIFTIGYAIQSRVTTEDPQNDFM 359
EHT+TE IT VD+V QI V++G SL + Q++I G AIQ RVTTEDP F
Sbjct: 332 EHTVTEEITDVDLVHAQIHVSEGRSLPDLGLR---QENIRINGCAIQCRVTTEDPARSFQ 388
Query: 360 PDTGKIMAYRSGGGFGVRLDTGNSFQGAVITPYYDSLLVKLSTWALTFEQAAAKMVRNLQ 419
PDTG+I +RSG G G+RLD ++FQGAVI+P+YDSLLVK+ AA KM R L
Sbjct: 389 PDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALA 448
Query: 420 EFRIRGIKTNIPFLENVAKHEKFLTGQYDTSFIDTTPELFNFPKQKDRGTKMLTYIGNVT 479
EFR+RG+KTNI FL+NV +++FL G DT FID PELF ++R K+L Y+G+V
Sbjct: 449 EFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVM 508
Query: 480 VNG-FPGIGKKEKPAFDKPLGVKVDVDQQPARGTKQILDEKGAEGLANWVKEQKSVLLTD 538
VNG I K P+ P+ V + PA G + IL +G EG A V+ +LL D
Sbjct: 509 VNGPTTPIPVKASPSPTDPVVPAVPIGPPPA-GFRDILLREGPEGFARAVRNHPGLLLMD 567
Query: 539 TTFRDAHQSLLATRIRSHDLKKIANPTAALWPELFSMEMWGGATFDVAYRFLKEDPWKRL 598
TTFRDAHQSLLATR+R+HDLKKIA A + +LFSME WGGATFDVA RFL E PW+RL
Sbjct: 568 TTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRL 627
Query: 599 EDLRKEVPNTLFQMLLRSSNAVGYTNYPDNVIKEFVKQSAQSGIDVFRIFDSLNWVKGMT 658
++LR+ +PN FQMLLR +NAVGYTNYPDNV+ +F + + ++G+DVFR+FDSLN++ M
Sbjct: 628 QELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNML 687
Query: 659 LAIDAVRDTGKVAEAAICYTGDILDKNRTKYDLAYYTSMAKELEAAGAHILGIKDMAGLL 718
L ++A G V EAAI YTGD+ D +RTKY L YY +A+EL AG HIL IKDMAGLL
Sbjct: 688 LGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLL 747
Query: 719 KPQAAYELVSALKETI-DIPVHLHTHDTSGNGIYMYAKAVEAGVDIIDVAVSSMAGLTSQ 777
KP A LVS+L++ D+P+H+HTHDTSG G+ +AG D++DVA SM+G+TSQ
Sbjct: 748 KPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQ 807
Query: 778 PSASGFYHAMEGNDRRPEMNVQGVELLSQYWESVRKYYSEFE--SGMKSPHTEIYEHEMP 835
PS G E+ ++ V S+YWE R Y+ F+ + MKS ++++YE+E+P
Sbjct: 808 PSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIP 867
Query: 836 GGQYSNLQQQAKGVGLGDRWNEVKEMYRRVNDMFGDIVKVTPSSKVVGDMALYMVQNNLT 895
GGQY+NL QA +GLG ++ EVK+ Y N M GD++KVTPSSK+VGD+A +MVQN L+
Sbjct: 868 GGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLS 927
Query: 896 EKDVYEKGESLDFPDSVVELFKGNIGQPHGGFPEKLQKLILKGQEPITVRPGELLEPVSF 955
+ + E L FP SVVE +G IG PHGGFPE + +LK + RPG L P+
Sbjct: 928 RAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDL 987
Query: 956 EAIKQEFKEQHNLEISDQDAVAYALYPKVFTDYVKTTESYGDISVLDTPTFFYGMTLGEE 1015
+A+++E ++H E++ +D ++ A+YP VF + T ++G + L+T F G + EE
Sbjct: 988 QALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEE 1047
Query: 1016 IEVEIERGKTLIVKLISIGEPQPDATRVVYFELNGQPREVVIKDESIKSSVQERLKADRT 1075
EVE+ERGKTL +K +++ + R V+FELNGQ R +++KD + KA +
Sbjct: 1048 FEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKD 1107
Query: 1076 NPSHIAASMPGTVIKVLAEAGTKVNKGDHLMINEAMKMETTVQAPFSGTIKQVHVKNGEP 1135
I A MPG VI + AG KV KG L + AMKMET V +P GT+++VHV
Sbjct: 1108 VKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMT 1167
Query: 1136 IQTGDLLLEIE 1146
++ DL+LEIE
Sbjct: 1168 LEGDDLILEIE 1178