Mycobacterium leprae

Genes

gi|15827047|ref|NP_301310.1| putative enolase [Mycobacterium leprae]
enolase 2; enolase-2, gamma, neuronal; neurone-specific enol

In addition, not only is that enolase, which catalyzes the dehydration reaction of Glycolysis, found as a protein in M. leprae, but is also present in human sperm. According to the European Journal of Cell Biology (2000, Februrary), enolase protein is detected "in tail fractions from mature sperm." The enzyme is also found in muscle and yeast and is important in the metabolism of carbohydrates. Enolase (2-phospho-D-glycerate hydrolase) is an essential glycolytic enzyme that catalyses the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is present as homodimers and heterodimers formed from alpa, beta, and gamma (three distinct subunits with the same molecular weight but which differ in their biochemical and immunological properties and tissue distribution). Alpha is a non-neuronal enolase that is found in many adult cell types. Beta is a musle-specific enolase that is found in muscle cells. Gamma is a neuron- specific anolase that is expressed exclusively in neurons.

Alignment

Score =      434 bits (1115), Expect = e-122
Identities = 237/428 (55%), Positives = 302/428 (70%), Gaps = 19/428 (4%)

Query: 22      IEQVGAREILDSRGNPTVEVEVVLIDGTFARAAVPSGASTGEYEAVELRDGDG-RYGGKG 80
               IE++ AREILDSRGNPTVEV++    G F RAAVPSGASTG YEA+ELRDGD  RY GKG
Sbjct: 3       IEKIWAREILDSRGNPTVEVDLYTAKGLF-RAAVPSGASTGIYEALELRDGDKQRYLGKG 61

Query: 81      VKRAVDAVLDEIGPVVI--GLNANDQRLIDQELLDLDGTPDKSRLGGNAILGVSLAVAKA 138
               V +AVD +   I P +I  GL+  +Q  +D  +L+LDGT +KS+ G NAILGVSLAV KA
Sbjct: 62      VLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKA 121

Query: 139     AADSAELPLFRYIG---GSNAHILPVPMMNILNGGAHADTAVDVQEFMVAPIGAPSFVEA 195
                A   ELPL+R+I    G++  ILPVP  N++NGG+HA   + +QEFM+ P+GA SF +A
Sbjct: 122     GAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDA 181

Query: 196     LRWGAEVYHALKSVLKKK--GLSTGLGDEGGFAPEVAGTTAALDLVTLAIEAAGFKPGAD 253
               +R GAEVYH LK V+K K    +T +GDEGGFAP +   + AL+LV  AI+ AG+
Sbjct: 182     MRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTE--K 239

Query: 254     VALALDAAATEFYTDG-IGYHFEGMTH-----TADQMTEFYADLLGSYPLVSIEDPLSED 307
               + + +D AA+EFY DG     F+  T      T DQ+   Y D +  YP+VSIEDP  +D
Sbjct: 240     IVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQD 299

Query: 308     DWDGWAALTASIGEQVQIVGDDIFATNPERLEEGIGRGVANALLVKVNQIGTLTETLEAV 367
               DW  W+  TA++G  +QIVGDD+  TNP+R+E  +      N LL+KVNQIG++TE ++A
Sbjct: 300     DWAAWSKFTANVG--IQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQAC 357

Query: 368     ALAHHSGYRTMISHRSGETEDTMIADLVVALGSGQIKTGAPARSERVAKYNQLLRIEEEL 427
                LA  +G+  M+SHRSGETEDT IADLVV L +GQIKTGAP RSER+AKYNQL+RIEEEL
Sbjct: 358     KLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEEL 417

Query: 428     GDAARYAG 435
               GD AR+AG
Sbjct: 418     GDEARFAG 425