CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: TP0837 592 aa Sequence 2: BB0720 581 aa Sequence 3: NMB0720 637 aa Sequence 4: XF0736 635 aa Sequence 5: HI1367 643 aa Sequence 6: thrS 642 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 43.8898 Sequences (1:3) Aligned. Score: 45.4392 Sequences (1:4) Aligned. Score: 45.777 Sequences (1:5) Aligned. Score: 45.6081 Sequences (1:6) Aligned. Score: 45.2703 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 39.759 Sequences (2:4) Aligned. Score: 38.7263 Sequences (2:5) Aligned. Score: 39.759 Sequences (2:6) Aligned. Score: 39.759 Sequences (3:2) Aligned. Score: 39.759 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 64.252 Sequences (3:5) Aligned. Score: 59.9686 Sequences (3:6) Aligned. Score: 60.7535 Sequences (4:2) Aligned. Score: 38.7263 Sequences (4:3) Aligned. Score: 64.252 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 57.4803 Sequences (4:6) Aligned. Score: 58.4252 Sequences (5:2) Aligned. Score: 39.759 Sequences (5:3) Aligned. Score: 59.9686 Sequences (5:4) Aligned. Score: 57.4803 Sequences (5:5) Aligned. Score: 100 Sequences (5:6) Aligned. Score: 78.0374 Sequences (6:2) Aligned. Score: 39.759 Sequences (6:3) Aligned. Score: 60.7535 Sequences (6:4) Aligned. Score: 58.4252 Sequences (6:5) Aligned. Score: 78.0374 Sequences (6:6) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 5 groups Aligning... Group 1: Sequences: 2 Score:11732 Group 2: Sequences: 2 Score:12741 Group 3: Sequences: 4 Score:11119 Group 4: Sequences: 5 Score:9549 Group 5: Sequences: 6 Score:8974 Alignment Score 31646 CLUSTAL-Alignment file created [clustal.aln]Clustal output
CLUSTAL W (1.81) multiple sequence alignment NMB0720 MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGRVNG-KLVDACDPIVEDSAVQI XF0736 MITITLPDGSRREFEGPVSVMQVAHAIGPGLAKATIAGQIDGGHLVDASDLIEHDAMLRI HI1367 MPIITLPDGSQRQFDRPVSVLEVAQDIGAGLAKATIAGRVNG-ERRDACYVIEQDATLEI thrS MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNG-ELVDACDLIENDAQLSI TP0837 ----------------------------------------MG---------LCVEENITM BB0720 ----------------------------------------------------------MS NMB0720 ITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGFYYDIATEKPFTPEDVAA XF0736 ITPEDQEALDIIRHSCAHLVGHAVKQLYPEAKMVIGPVIADGFYYDIYSKRPFTPEDLAA HI1367 ITAKDEDGLEIIRHSCAHLLGHAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDA thrS ITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGFYYDVDLDRTLTQEDVEA TP0837 LQ-KRSDTLDRLRHSLAHVMAEAVQALFPGTKLAVGPPIDYGFYYDFSPPRPLCDADLAP BB0720 KDLDKEDILYKKRHSIAHVMAEAVLDLFPNTKIAIGPPIKDGFYYDFEFKKQITEDSLLD . .: : *** **::..*: *:* .*:.:** * *****. : : .: NMB0720 IEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLIDDM-PEVEAMGMYHHQEYV XF0736 IEQRMVELIAQDYHVVKHITARHDVVRLFKERGEDYKLRLIEEMGPEVTAMGIYHHQEYV HI1367 IEKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAILDENIERTATPALYHHLEYI thrS LEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSILDENIAHDDKPGLYFHEEYV TP0837 IEEKMRAILRAGCPFVKEVVSRPDALARFKD--EPFKQELIERISADDT-LSLYHSGAFT BB0720 IENRMREILKTGSSFEKEIISVEQALEIFKD--EPYKIDLIKNFDLQNE-VSIYKSHNFV :* :* : . . * . :. * . * :* ::. .:* : NMB0720 DMCRGPHVPNTRFL--KNFKLTKLAGAYWRGDSNNEMLQRIYGTAWATKDELKAYIQRIE XF0736 DMCRGPHVPNTRFL--KAFKLTRISGAYWRGDTKNEQLQRIYGTAWADKKQLEAHMQRLQ HI1367 DMCRGPHVPNMRFC--QHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLE thrS DMCRGPHVPNMRFC--HHFKLMKTAGAYWRGDSNNKMLQRIYGTAWADKKALNAYLQRLE TP0837 DLCRGPHVQSMRDINPHAFKLTSIAGAYWRGNERGPQLTRIYGTAWESEEDLHTYLRMQD BB0720 DLCRGPHVENMNKIDPKAFKLTSIAGAYWRGSEKNPMLTRIYGTLWNNEKELRSYLNLRE *:****** . . : *** :******. .. * ***** * :. * :: : NMB0720 EAEKRDHRKLGKQLDLFHLQDEA-PGMVFWHPKGWALWQVIEQHMRKELNAAGYKEVKTP XF0736 DAEKRDHRKIGKVQDLFHLQEEG-PGLVFWHPKGWVIWQTIENYIRRVYRNSGYGELRCP HI1367 EAAKRDHRKIGKALDLYHMQEEA-PGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGP thrS EAAKRDHRKIGKQLDLYHMQEEA-PGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGP TP0837 EAKRRDHRKLGPALGLFHLDEEN-PGQVFWHPEGWTLYVAIQQYLRRVMHEDGYAEVHTP BB0720 EIKKRDHRKLGKELDLFSIHEEIGPGLVFFHPNGAKIRALIEDFWREEHSKNGYDILFTP : :*****:* .*: :.:* ** **:* .* : :: . * * : * NMB0720 QIMDKTFWEKSGHWDNYKDNMFV-TSSEKREYAVKPMNCPGHVQIFNNGLRSYRDLPMRL XF0736 QILDVSLWQKSGHWDNYKENMFF-TDSEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRY HI1367 FMMDRVLWEKTGHWQNYADLMFT-TQSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRM thrS FMMDRVLWEKTGHWDNYKDAMFT-TSSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRM TP0837 FVMPQSLWERSGHWDKYRANMYL-TEGEKRSFALKPMNCPGHVEIFKQKTRSYRDLPLRL BB0720 HIGKSWLWQTSGHLDFYKDSMFEKIEMDKSDYYLKPMNCPFHIAIYNTGKHSYRDLPFRW : :*: :** : * *: . :: : :****** *: ::: :******:* NMB0720 AEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQIVSEARAFNELLIRIYKQFGF-- XF0736 GEFGACHRNEPSGALHGLLRVRGFTQDDGHIFCTEAQIESEVTAFHRQALQVYADFGF-- HI1367 AEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCIKMVYDIYSTFGF-- thrS AEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQIRDEVNGCIRLVYDMYSTFGF-- TP0837 SEFGSCTRNEPSGSLHGVMRVRGFVQDDAHIFCTEAQIASEVTRFCRLLARVYADFGFAQ BB0720 AELGTVYRYEKIGALHGMMRARGFTQDDAHIICTHSQVLDEIKEVLRFAIYMWSKFGF-- .*:*: * * *:***::*.***.***.**:**. *: .* . :: *** NMB0720 HDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVEWGELPGEGAFYGPKIEYHVRDA XF0736 EDIQIKIALRPDKRLGDSLSWDKAEAALRAALSACEVEWQELPGEGAFYGPKIEYHLKDA HI1367 TNIAVKLSTRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDC thrS EKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNIPFEYQLGEGAFYGPKIEFTLYDC TP0837 EQIRVKFSTRPEQRIGDDATWDRAERALAEACEAAGLSYEHAPGEGAFYGPKLEFALIDT BB0720 SNPKAYLSTKPDKSVGNDSDWEMSLKVLEETLSDFEVPYEIDKGGGAFYGPKIDLKIVDS . :: :*:: *.. *: : * : : : * *******:: : * NMB0720 LGRSWQCGTLQLDFVLP--ERLNAEYVTENNDRARPVMLHRAILGSLERFIGILIENHAG XF0736 IGRTWQLGTIQVDFMMP--GRLGAEYVDERSQRCHPVMLHRAIVGSMERFIGILIEHYAG HI1367 LGREWQCGTVQLDFALP--GRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAG thrS LDRAWQCGTVQLDFSLP--SRLSASYVGEDNERKVPVMIHRAILGSMERFIGILTEEFAG TP0837 LEREWQCGTIQVDYQLPSCERLNAEYVGEDNQRHMPVILHRTVIGSLERFIGILIEHYGG BB0720 LEREWQMSTIQFDFNLP--ERFNMTYTAEDGKEKRPFMIHRALLGSIERFFGILVEHYGG : * ** .*:*.*: :* *:. *. * ... *.::**:::**:***:**: *...* NMB0720 SFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGFRAELDLRNEKIGYKIRDNSQYRFP XF0736 IWPTWLAPVQVVVANITDAQYEYAERVHKALLNQGFRVNTDLRNEKIGYKIREHTLQRVP HI1367 FFPAWLAPTQAVVMNITDSQADYVQKVAKQLSDVGLRVKTDLRNEKVGFKIREHTLRRVP thrS FFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVP TP0837 AFPPWLAPVQAVVIPVAPAFLEYAQHVARELCARSLRVQADVSAERMNAKIRTAQTQKVP BB0720 AFPLWLSPVQVVIIPVNNIVEDYAIKVFNKFKNEGIRIKLDNSSSRMNAKIREYQAKKIP :* **:*.* *: : :* .: : .:* : * .::. *** :.* NMB0720 YQIVVGDKEKQENKVAVR-RKAEDLGSLDLDDFIAQLQQEITDALVNH---- XF0736 YLLVVGDREKENGTVAVRTCSREDLGAMSISAFVERLQAEQVV--------- HI1367 YMLVCGDKEIAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLLNEE thrS YMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEVIEKLQQEIRSRSLKQLEE- TP0837 YLLIVGERELRAQQVAVRPRTGP-QHSMGLSAFSTFLLAKLETRALHA---- BB0720 YMFIIGEREATEERISIRTRTNEQINGMKLDEALKFILFKIRDKEI------ * :: *::* :::* : :. : :Tree construction
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: NMB0720 652 aa Sequence 2: XF0736 652 aa Sequence 3: HI1367 652 aa Sequence 4: thrS 652 aa Sequence 5: TP0837 652 aa Sequence 6: BB0720 652 aa Phylogenetic tree file created: [clustal.ph]+--------------------------1:NMB0720 | --7 +----------------------------2:XF0736 | | +--8 +-----------------3:HI1367 | +-------------10 | | +----------------4:thrS +----9 | +-------------------------------------5:TP0837 +----------11 +------------------------------------------------6:BB0720Midpoint-rooted tree:
+------------------------------------1:NMB0720 +------8 | +---------------------------------------2:XF0736 +-----------------9 | | +-------------------------3:HI1367 +-11 +-------------------10 | | +------------------------4:thrS --7 | | +-----------------------------------------------------5:TP0837 | +------------------------------------------------------------------6:BB0720Remember, this is an unrooted tree!
Bootstrapping tree
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: NMB0720 652 aa Sequence 2: XF0736 652 aa Sequence 3: HI1367 652 aa Sequence 4: thrS 652 aa Sequence 5: TP0837 652 aa Sequence 6: BB0720 652 aa Phylogenetic tree file created: [clustal.ph]