CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: alaS 876 aa Sequence 2: CC2529 880 aa Sequence 3: HI0814 874 aa Sequence 4: NMB1595 874 aa Sequence 5: VC0545 860 aa Sequence 6: XF0124 916 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 48.8584 Sequences (1:3) Aligned. Score: 68.9931 Sequences (1:4) Aligned. Score: 59.9542 Sequences (1:5) Aligned. Score: 68.6047 Sequences (1:6) Aligned. Score: 54.1096 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 48.2838 Sequences (2:4) Aligned. Score: 47.0252 Sequences (2:5) Aligned. Score: 44.0698 Sequences (2:6) Aligned. Score: 47.3864 Sequences (3:2) Aligned. Score: 48.2838 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 56.1785 Sequences (3:5) Aligned. Score: 63.7209 Sequences (3:6) Aligned. Score: 51.1442 Sequences (4:2) Aligned. Score: 47.0252 Sequences (4:3) Aligned. Score: 56.1785 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 57.7907 Sequences (4:6) Aligned. Score: 50 Sequences (5:2) Aligned. Score: 44.0698 Sequences (5:3) Aligned. Score: 63.7209 Sequences (5:4) Aligned. Score: 57.7907 Sequences (5:5) Aligned. Score: 100 Sequences (5:6) Aligned. Score: 49.186 Sequences (6:2) Aligned. Score: 47.3864 Sequences (6:3) Aligned. Score: 51.1442 Sequences (6:4) Aligned. Score: 50 Sequences (6:5) Aligned. Score: 49.186 Sequences (6:6) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 5 groups Aligning... Group 1: Sequences: 2 Score:15945 Group 2: Sequences: 3 Score:15909 Group 3: Sequences: 4 Score:15002 Group 4: Sequences: 5 Score:14449 Group 5: Sequences: 6 Score:13808 Alignment Score 45539 CLUSTAL-Alignment file created [clustal.aln]Clustal output
CLUSTAL W (1.81) multiple sequence alignment alaS ------------------------------------MSKSTAEIRQAFLDFFHSKGHQVV VC0545 ------------------------------------MFMSTDEVRRAFLSFFESKGHQIV HI0814 -------------------------------------MKTTAEIRQSFLDFFHSKGHQVV NMB1595 --------------------------------------MKTSELRQKFLKFFETKGHTVV XF0124 MNVQCLLPLIDLRPYPHSVRLARLQCQPTVNSMNIPTKFTTSKIRSDFLEFFKNKGHKIV CC2529 -------------------------------------MPSLNEIRSTFLDYFGKADHAVT . ::* **.:* . .* :. alaS ASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYT VC0545 ESSSLVPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTAQRCVRAGGKHNDLENVGFT HI0814 ESSSLVPENDPTLLFTNAGMNQFKDVFLGMDKRPYSRATTAQRCVRPGGKHNDLENVGYT NMB1595 RSSSLVPHDDPTLLFTNAGMNQFKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYT XF0124 PSAPLVPGNDPTLLFTNSGMVQFKDVFLGAEKRSEVRVADVQCCLRAGGKHNDLDSVGYT CC2529 PSAPLVPQNDPTLLFVNAGMVPFKNVFTGAETPAHPRAASSQKCVRAGGKHNDLDNVGYT *:.*** :******.*:** **: * * :. *.: * *:*.*******:.**:* alaS ARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWE VC0545 ARHHTFFEMLGNFSFGDYFKEDAIQYAWEFLT--DVLQLPKERLLVTVYETDDEAFDIWN HI0814 ARHHTFFEMLGNFSFGDYFKQDAINFAWEYLTSPQWLGLPKEKLWVTVYETDDEAYNIWN NMB1595 ARHHTFFEMMGNFSFGDYFKRDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWL XF0124 ARHHTFFEMLGNWSFGDYFKKEAITWAWELLT--HVWELPPERLLVTVYHTDDESYALWR CC2529 ARHHTFFEMLGNFSFGDYFKEQAILHAWTLLT--AEFKLPKDKLLVTVYHTDDEAADLWK *********:**:*******.:** ** ** :* ::* .*** ***: :* alaS KEVGIPRERIIRIGDNKGAP-YASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEE VC0545 KKVGIPADRIIRIGDKKGGKKFDSDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEE HI0814 KDVGVPAERIIRIGDNKGSP-YASDNFWAMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEE NMB1595 NEIGMPSERIVRIGDNKGAK-YASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEE XF0124 DMVGVPEDRIVRIGDNKGAP-FASDNFWQMADTGPCGPCTEIFYDHGEHIPGGPPGSPDE CC2529 KIAGLGDDRIIRIP--------TSDNFWSMGDTGPCGPCSEIFFDHGPHIAGGPPGSPDE . *: :**:** ***** *.********:***:*** .* ** ****:* alaS DGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTL VC0545 DGDRFIEIWNNVFMQFNRHADGTMEPLPKPSVDTGMGIERISAIMQGVHSNYEIDVFQTL HI0814 DGDRYIEIWNVVFMQFNRLADGTMEKLPRPSVDTGMGLERISAVLQHVNSNYEIDIFKTL NMB1595 DGDRWIEIWNCVFMQFNRDEQGNMNPLPKPSVDTGMGLERIAAVMQHVHSNYEIDLFQDL XF0124 DGDRFIEIWNLVFMQFDRQSDGTLVPLPAPCVDTGMGLERLAAILQHVHTNYEIDLFQTL CC2529 DGDRFIEIWNLVFMQFEQLAGGERISLPKPSIDTGMGLERIAAVLQGQHDNYDIDLFKAL ****:***** *****:: * ** *.:*****:**::*::* : **:**:*: * alaS IQAVAKVTGATDLSNK--SLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHG VC0545 IKAAADAIGYQDLTNQ--SLRVVADHIRSCAFLIVDGVMPSNEGRGYVLRRIIRRAVRHG HI0814 IAKTAEIVGATDLTNK--SLRVIADHIRSCAYLIADGVIPSNEGRGYVLRRIIRRAVRHG NMB1595 LKAVARETGAPFRMEEP-SLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHG XF0124 ILKAAELTAVADVQNK--SLRVIADHSRACAFLIVDGVLPSNEGRGYVLRRIIRRALRHG CC2529 IAASVELTGVKAEGAQAPSHRVIADHLRSSSFLLADGVSPSNEGRGYVLRRIMRRAMRHA : . . : * :*:*** *:.::*:.*** ****.********:***:**. alaS NMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEE VC0545 NKLGAQGAFFHKLVGPLAEVMGTAGVELKKQQALVEKVLRIEEENFGRTLDRGMSILNDA HI0814 HLLGAKESFFYKLVPTLIDVMAEAGKDVKAKQTNVEKLLRLEEEQFARTLERGLSLLDEA NMB1595 YKLGQSKPFFHKLVADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENA XF0124 WMLGVRQPFFNNMVPTLVAVMGDAYPKLQAAAESVMRTLLAEEERFAETLDVGMKIFNEV CC2529 YLLGASEPLMHRLAPTLVREMGQAYPELRRAEALIVETLRQEEERFRTTLGRGMGLLDEA ** .:: .:. * *. * .:: : . * **..* ** *: :::: alaS LAKLS-GDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRARE-- VC0545 LDQLS-GQVLDGETVFKLYDTYGFPADLTNDVARERGFSIDEAGFEQAMEEQRQRARE-- HI0814 LSQVK-DGILSGEVAFKLYDTYGFPLDLTADVCRERNITIDEQAFDRKMEAQRTRAQA-- NMB1595 LVKG--GKTLGGEIIFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARA-- XF0124 AAKVA-NGVIPGSDAFRLYDTYGFPVDLTADIARERGMSVDMVGFEAAMTQQRKTARA-- CC2529 TANLAAGGVLDGETAFKLYDTYGFPLDLTQDAVRAKGLTVDTDAFDAAMQKQREMARANW : . : *. *:******** *** * * :.: * .*: * ** *: alaS --ASGFGADYNAMIRVD---SASEFKGYDHLELN-GKVTALFVDGKAVDAINAGQEAVVV VC0545 --AGQFGTDYNSLIKSA---TNTEFCGYTASRGQ-SVVREMFVEGAEVSTLSAGDKAIIV HI0814 --ASQFGVDYNSVIRVD---GETKFEGYTEVESQ-AKITALFYDGKSVESIEAGQSAVVI NMB1595 --AQSFKAN-AQLPYDG---QDTEFKGYSERQTE-SKVLALYKDGEQVNELNEGDSGAVV XF0124 --AGKFGRGVQLSAERAAMLSPTVFLGYEQLQADGLRVVALLSDGGLTDSASVGDEVIVL CC2529 AGSGQQGAAAAWFSIKEK-AGATDFVGYETTETT-GVVKAIVVDGAEVESASAGQTVEVL : : * ** . : : :* .. . *: :: alaS LDQTPFYAESGGQVGDKGELKGAN-FSFAVEDTQKYG-QAIGHIGKLAAGSLKVGDAVQA VC0545 LDNTPFYAESGGQCGDTGVLKTDA-GIFHVEDTQKLG-NAIAHHGVIAQGVLATGDQVDA HI0814 LENTPFYAESGGQIGDSGYLSTQS-VTFNVKDTQKYG-QVFGHIGELTQGSLKVGQSVNA NMB1595 IDFTPFYAESGGQVGDVGYIFSGE-NRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTA XF0124 TDRTPFYAESGGQVGDIGTLMASDGVRLEVTDTQKLMGQFHGHVARIVQGGVKVGDVLSG CC2529 LDRTSFYAESGGQAGDTGVIEAHG-RESRVLDTQKQAGELHVHRVELAS-DLKVGDQVVA : *.******** ** * : * **** : . : .*: : . alaS DVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIR VC0545 IVDEKRRAAISLNHSATHLLHAALRKVLGEHVAQKGSLVRAETLRFDFSHLEAMTAAEIK HI0814 IVDAKRRHNTSLNHSATHLLHAALRQILGLHVVQKGSLVSDKALRFDFAQPEAITKEQLS NMB1595 KVDDEIRNANMRNHSATHLMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIA XF0124 SVAVARRKMVALNHSATHLLHCALRSVFGTHVVQKGSLVAPDRLRFDFSHFEPIAAAQMT CC2529 SVDADRRNTTRSNHSAAHLVHAALHHVLGPHVAQKGQMVDGERMRFDFSHGGPLTAEELD * * ****:**:* **: ::* ** ***.:* . ***::: .: :: alaS AVEDLVNTQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGD--------FS VC0545 EVERLVNQEVRRNHSIETNIMNIDEAKAKGAMALFGEKYDDQVRVLSMGD--------FS HI0814 EIETLVNQKIRANFPVQTDIMDIDSAKAKGAMALFGEKYGDKVRVLTMGD--------FS NMB1595 EVERRVNEAILANVAVNAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGG--------FS XF0124 LIERMVNDEVRANHLVMIEQMGMQAALDAGAMALFGEKYGEHVRVVTMG---------TS CC2529 RIEAEVNEVIRQNVPAETKEMAPQEAIEAGAVALFGEKYGDSVRVLTLGKSLADEAKAYS :* ** : * * : * **: ******.: ***: :* * alaS TELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLK VC0545 TELCGGIHASNTGDIGLFKIISEGGIAAGIRRIEAVTGEGALDYLDAQQAQH-------- HI0814 IELCGGIHAKRTGDIGLFKIITENAVAAGIRRIEAVTGQNAIDWLHNQQRILTQSADLLK NMB1595 TELCGGTHVSRTGDIGLFKIISEGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETK XF0124 VELCGGTHINRTGDIGLFKIISECGVSSGVRRIEAVTGESALNHVLAEEHRLYEVAGLIG CC2529 VELCGGTHVARTGDIALFKIVSEQGVAAGVRRIEALTGEAARRFLLDQAGVAKALADQFK ***** * .****.**:*::* . ::*:*****:** * : alaS GDSN-NLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGV VC0545 -------DAKVSEMAAKAKLLEKEIQQLKDKLAAKESAGLINQVKQIAGVNVLVAQLNGA HI0814 SDVN-TLAEKIQQLQDKAKKVEKELQGLKEKAAMQAGSYFVKSAVKINGVSVIAQQLDGI NMB1595 AQTEKDVLAKIQAGAAHAKALEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIE-A XF0124 SNAN-DVVNHIRQLTDRQKTLERELEKLKGKLISGTITDLLSTAVNVADVKVVAARLDGL CC2529 TPVA-EVATRVDALIADRKRLEKELAEAKKQLALGGGG-AASGPEDVNGTALIARVLDGV :: : :*:*: * : . .: . ::. :. alaS EPKMLRTMVDDLKNQLGSTIIVLATVVEGKVSLIAGVS-KDVTDRVKAGELIGMVAQQVG VC0545 DNKALRGMVDDLKNQLSSGIIMLGNVAEGKVGLIAGVT-NDLTNKVKAGELVNMVALQVG HI0814 ETKSLRVMVDDLKNQLGSGVIAFASILDEKVNLVVGVT-NDLTAKIKAGELVNLMAQQVG NMB1595 DAAALREIVTDLTGKSDNAVILLAAVNDGKVSLCAGVS-KPLTGKVKAGDLVKFAAEQVG XF0124 DGKALREALDRLKLQLSDAVIVLAGVTGGKVALVTAVNGRRAMGKVKADTLLSHVATQIN CC2529 GGKELRGVAEEFKKQLASGVVALVGTSDGKAAVTVAVT-ADLTGKFSAADLAKAAVIAMG ** :. : . :: : *. : ..*. :..* * . :. alaS GKG-GGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ VC0545 GKG-GGRPDMAQAGGTDAHALPSALESVDAWIAERL- HI0814 GKG-GGRPDMAMAGGSQLENVTQAIKVAQDWLNKNL- NMB1595 GKG-GGRPDLAQAGGTDADKLPAVLDSVKDWVGAKLV XF0124 GRG-GGRVDFAQGGGEDGPSLRSALDGVATWVKQHLN CC2529 GQGAGGKADFAQGGAPDDSKAEEGLAAVKALLAG--- *:* **: *:* .*. : : . :Tree construction
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: alaS 937 aa Sequence 2: VC0545 937 aa Sequence 3: HI0814 937 aa Sequence 4: NMB1595 937 aa Sequence 5: XF0124 937 aa Sequence 6: CC2529 937 aa Phylogenetic tree file created: [clustal.ph]+----------------------------1:alaS +--8 | +------------------------------------2:HI0814 --7 | +---------------------------------3:VC0545 +--9 | +-------------------------------------------4:NMB1595 +---10 | +-----------------------------------------------5:XF0124 +-----11 +-----------------------------------------------------6:CC2529Midpoint-rooted tree:
+------------------------------------1:alaS +--8 +-----9 +----------------------------------------------2:HI0814 | | +-------10 +-------------------------------------------3:VC0545 | | +-11 +-------------------------------------------------------4:NMB1595 | | --7 +-------------------------------------------------------------5:XF0124 | +------------------------------------------------------------------6:CC2529Remember, this is an unrooted tree!
Bootstrapping tree
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: alaS 937 aa Sequence 2: VC0545 937 aa Sequence 3: HI0814 937 aa Sequence 4: NMB1595 937 aa Sequence 5: XF0124 937 aa Sequence 6: CC2529 937 aa Phylogenetic tree file created: [clustal.ph]