CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: gi|15838454|ref|NP_299142.1| 589 aa Sequence 2: gi|15602848|ref|NP_245920.1| 588 aa Sequence 3: gi|15641179|ref|NP_230811.1| 591 aa Sequence 4: gi|15794899|ref|NP_284721.1| 602 aa Sequence 5: gi|15676377|ref|NP_273513.1| 602 aa Sequence 6: gi|15596160|ref|NP_249654.1| 591 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 50.3401 Sequences (1:3) Aligned. Score: 51.1036 Sequences (1:4) Aligned. Score: 50.5942 Sequences (1:5) Aligned. Score: 50.0849 Sequences (1:6) Aligned. Score: 48.5569 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 73.4694 Sequences (2:4) Aligned. Score: 55.4422 Sequences (2:5) Aligned. Score: 55.102 Sequences (2:6) Aligned. Score: 61.7347 Sequences (3:2) Aligned. Score: 73.4694 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 55.4992 Sequences (3:5) Aligned. Score: 55.6684 Sequences (3:6) Aligned. Score: 61.0829 Sequences (4:2) Aligned. Score: 55.4422 Sequences (4:3) Aligned. Score: 55.4992 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 98.6711 Sequences (4:6) Aligned. Score: 60.5753 Sequences (5:2) Aligned. Score: 55.102 Sequences (5:3) Aligned. Score: 55.6684 Sequences (5:4) Aligned. Score: 98.6711 Sequences (5:5) Aligned. Score: 100 Sequences (5:6) Aligned. Score: 60.5753 Sequences (6:2) Aligned. Score: 61.7347 Sequences (6:3) Aligned. Score: 61.0829 Sequences (6:4) Aligned. Score: 60.5753 Sequences (6:5) Aligned. Score: 60.5753 Sequences (6:6) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 5 groups Aligning... Group 1: Sequences: 2 Score:11317 Group 2: Sequences: 3 Score:10580 Group 3: Sequences: 2 Score:13001 Group 4: Sequences: 5 Score:10262 Group 5: Sequences: 6 Score:9727 Alignment Score 34336 CLUSTAL-Alignment file created [clustal.aln]Clustal output
CLUSTAL W (1.81) multiple sequence alignment gi|15602848|ref|NP_245920.1| MMRTHYCGALNRDNIGQEVTLSGWVHRRRDLGGLIFIDMRDREGIVQVCF gi|15641179|ref|NP_230811.1| -MRSHYCGHLNKSLVGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVV gi|15596160|ref|NP_249654.1| MMRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVF gi|15794899|ref|NP_284721.1| -MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGIVQVVI gi|15676377|ref|NP_273513.1| -MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGIVQVVI gi|15838454|ref|NP_299142.1| -MRTHFCGLIDETLIGHTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTV **:::** :.. .: * : **.. *: **: *:*:**:**:.** . gi|15602848|ref|NP_245920.1| DP-----VYQEALTTAASLRNEFCIQIKGEVIARPDNQINKNMATGEVEV gi|15641179|ref|NP_230811.1| DP-----DMADVFAVANQLRSEFCIKLTGEVRARPESQVNKEMATGEVEL gi|15596160|ref|NP_249654.1| DP-----DRAETFAKADRVRSEFVVKITGKVRLRPEGARNPNMASGSIEV gi|15794899|ref|NP_284721.1| DP-----DTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEI gi|15676377|ref|NP_273513.1| DP-----DTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEI gi|15838454|ref|NP_299142.1| DSRAIDQNNSELFKVASGLSYEDVLQVEGVVCAR--HAVNDKIKTGKVEV *. : : * * :.: * * * * :: :*.:*: gi|15602848|ref|NP_245920.1| LAKSLSIYNSAE--PLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRA gi|15641179|ref|NP_230811.1| LARSLEIINRSD--VLPLDFNQKNSEEQRLKYRYLDLRRPEMSDRIKLRA gi|15596160|ref|NP_249654.1| LGYELEVLNQAETPPFPLDEYSDVGEETRLRYRFIDLRRPEMAAKLKLRA gi|15794899|ref|NP_284721.1| LAKEIEVLNAAATPPFQIDD-ENISENVRLTNRVIDLRRPVMQRNLRLRY gi|15676377|ref|NP_273513.1| LAKEIEVLNAAATPPFQIDD-ENISENVRLTNRVIDLRRPVMQRNLRLRY gi|15838454|ref|NP_299142.1| IATKIKILNKAA--PLPFHAHENPGEDIRLKYRYLDLRRPEMQRMQRTRI :. .:.: * : : :. .. *: ** * :***** * : * gi|15602848|ref|NP_245920.1| KITSFVRRFMDEHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQ gi|15641179|ref|NP_230811.1| KASSFVRRFLDTHGFLDIETPVLTKATPEGARDYLVPSRVHKGSFYALPQ gi|15596160|ref|NP_249654.1| RITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLVPSRTYPGHFFALPQ gi|15794899|ref|NP_284721.1| QVAMGVRRYLDAQGFIDIETPMLTRSTPEGARDYLVPSRVHPGEFFALPQ gi|15676377|ref|NP_273513.1| QVAMGVRRYLDAQGFIDIETPMLTRSTPEGARDYLVPSRVHPGEFFALPQ gi|15838454|ref|NP_299142.1| KLVQALRRHLDMHGFQDIETPILTKATPEGARDFLVPARMHPGEFYALPQ : :**.:* :** *:***:* :.*******:***:* : * *:**** gi|15602848|ref|NP_245920.1| SPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFLTAP gi|15641179|ref|NP_230811.1| SPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMTAE gi|15596160|ref|NP_249654.1| SPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSFLDES gi|15794899|ref|NP_284721.1| SPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDLETSFLNED gi|15676377|ref|NP_273513.1| SPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDLETSFLNED gi|15838454|ref|NP_299142.1| SPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFAFVSER *******:**::********.:***** ****** ****:*:* :*: gi|15602848|ref|NP_245920.1| EVREIMEKMVHGLWRETLGVDLG-KFPVMTFAEAMRRFGSDKPDLRNPLE gi|15641179|ref|NP_230811.1| QVRAVTEKMIREMWLELLNVDLG-DFPIMPYSEAMRRFGSDKPDLRNPME gi|15596160|ref|NP_249654.1| DIIGITEKMVRQLFKEVLDVEFD-EFPHMPFEEAMRRYGSDKPDLRIPLE gi|15794899|ref|NP_284721.1| EIMDITEGMAKQVFKDALGVDLG-DFPRMPYSEAMFYYGSDKPDMRINLK gi|15676377|ref|NP_273513.1| EIMDITEGMAKQVFKDALNVDLG-DFPRMPYSEAMFYYGSDKPDMRINLK gi|15838454|ref|NP_299142.1| DVQDFVEEMIRRVFKEVAGIELDTTFPRMTWTEAMRRFGSDKPDLRINLE :: . * * : :: : .:::. ** *.: *** :******:* :: gi|15602848|ref|NP_245920.1| MVDVADLLKEVEFKVFNGPANDPNGRIAVIRVPNGTDITRKQIDEYTQFV gi|15641179|ref|NP_230811.1| LVDVADLLKDVDFKVFSGPANDPKGRVAALCIPGGAALTRKQIDEYTAFV gi|15596160|ref|NP_249654.1| LVDVADQLKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFV gi|15794899|ref|NP_284721.1| FTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKEIDEYTKFV gi|15676377|ref|NP_273513.1| FTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAEFSRKEIDEYTKFV gi|15838454|ref|NP_299142.1| LIDVAALVADSTFTPFTDAVAHPNGRVAALRIPSGAVLSRKQIDEYAAYT : ::: : *. * ... **:..: :*..: :.*.:**:*: :. gi|15602848|ref|NP_245920.1| GIYGAKGLAWLKVNDLAAGMDG----VQSPIAKFLSEAVLKALLERVQAQ gi|15641179|ref|NP_230811.1| AIYGAKGLAWLKVNDLAAGMEG----IQSPVAKFLTEEIIQAIIERTQAQ gi|15596160|ref|NP_249654.1| GIYGAKGLAYIKVNERAKGVEG----LQSPIVKFIPEANLNVILDRVGAV gi|15794899|ref|NP_284721.1| GIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ gi|15676377|ref|NP_273513.1| GIYGAKGLAYIKVNDVSNLSNGEDSGLQSPIVKYLSENALKEIIARTGAQ gi|15838454|ref|NP_299142.1| AKYGATGLAYAKLAPIG--------EITSPIAKFFSEDAFAALLSHIGAE . ***.***: *: . : **:.*::.* : :: : * gi|15602848|ref|NP_245920.1| NGDILFFGADNYKVTTDAMGALRLKLGRDLG---LTKLEEWQPLWVIDFP gi|15641179|ref|NP_230811.1| TGDIILFGADSAKVVAEALGALRLKAGKELG---ITNESAWAPLWVVDFP gi|15596160|ref|NP_249654.1| DGDIVFFGADKAKIVCDALGALRIKVGHDLK---LLTR-EWAPMWVVDFP gi|15794899|ref|NP_284721.1| NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFP gi|15676377|ref|NP_273513.1| NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFP gi|15838454|ref|NP_299142.1| KGDIVFFGAGNYNKVSDFMGALRLKAGKDFA----LITADWRPLWVTDFP ***::***.. : . : :****:* * : * *:** *** gi|15602848|ref|NP_245920.1| MFERDDEGD-LAAMHHPFTSPKDFSPE-QLEADPTSAVANAYDMVINGYE gi|15641179|ref|NP_230811.1| MFESDDEGN-VAAMHHPFTSPLNLSPE-QLKANPEEALSNAYDMVLNGYE gi|15596160|ref|NP_249654.1| MFEENDDGS-LSALHHPFTSP-KCTPA-ELEANPGAALSRAYDMVLNGTE gi|15794899|ref|NP_284721.1| MFEYDEEADRYVAVHHPFTAPKEGHED-LMVSDPANCLARAYDMVLNGWE gi|15676377|ref|NP_273513.1| MFEYDEEADRYVAVHHPFTAPKEGHED-LMVSDPANCLARAYDMVLNGWE gi|15838454|ref|NP_299142.1| MFEWDEEAQRYVALHHPFTAPAAIDDIDELRAHARTALSRGYDMVLNGNE *** :::.. *:*****:* : :.. .::..****:** * gi|15602848|ref|NP_245920.1| VGGGSVRIFDPKMQQTVFRILGINEAEQREKFGFLLDALKFGTPPHAGLA gi|15641179|ref|NP_230811.1| VGGGSVRIHNAEMQSAVFDILGITPEEQRLKFGFLLDALKFGTPPHAGLA gi|15596160|ref|NP_249654.1| LGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLA gi|15794899|ref|NP_284721.1| IGGGSIRIHRADVQEKVFAALKISPEEQQEKFGFLLDNLKFGAPPHGGLA gi|15676377|ref|NP_273513.1| IGGGSIRIHRADVQEKVFAALKISPEEQQEKFGFLLDNLKFGAPPHGGLA gi|15838454|ref|NP_299142.1| IGGGSIRIHRPEMQRAVFELLGITEDEARAKFGFLLDALNYGAPPHGGIA :****:**. .:* ** * * * . ******* *::*:***.*:* gi|15602848|ref|NP_245920.1| FGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPQALNELSVQ gi|15641179|ref|NP_230811.1| FGLDRLVMLLCGTENIRDVIAFPKTTAAACLMTDAPSLANPAALEELAIA gi|15596160|ref|NP_249654.1| FGLDRLVMLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIR gi|15794899|ref|NP_284721.1| FGLDRLVTLMTGAESIRDVIAFPKTQRAQCLLTNAPNSVDDKQLRELSLR gi|15676377|ref|NP_273513.1| FGLDRLVTLMTGAESIRDVIAFPKTQRAQCLLTNAPNSVDDKQLRELSLR gi|15838454|ref|NP_299142.1| FGIDRIAALIAGTESIRDVIPFPKTTGAQCLMTDAPSSISEEQLSEIHVI **:**:. *: *:..**:**.**** * ::*:**. . * *: : gi|15602848|ref|NP_245920.1| VIKSE------ gi|15641179|ref|NP_230811.1| VKLATKDKA-- gi|15596160|ref|NP_249654.1| LREQPKAE--- gi|15794899|ref|NP_284721.1| LRQKAAETKEA gi|15676377|ref|NP_273513.1| LRQKATETKEV gi|15838454|ref|NP_299142.1| TKKPTP-----Tree construction
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|15602848|ref|NP_245920.1| 611 aa Sequence 2: gi|15641179|ref|NP_230811.1| 611 aa Sequence 3: gi|15596160|ref|NP_249654.1| 611 aa Sequence 4: gi|15794899|ref|NP_284721.1| 611 aa Sequence 5: gi|15676377|ref|NP_273513.1| 611 aa Sequence 6: gi|15838454|ref|NP_299142.1| 611 aa Phylogenetic tree file created: [clustal.ph]+----------------1:gi|15602848|ref|NP_245920.1| +-------9 +--8 +----------------2:gi|15641179|ref|NP_230811.1| | | --7 +----------------------------------3:gi|15838454|ref|NP_299142.1| | | +-----------------------4:gi|15596160|ref|NP_249654.1| +-10 | +--5:gi|15794899|ref|NP_284721.1| +------------------------11 +--6:gi|15676377|ref|NP_273513.1|Midpoint-rooted tree:
+-------------------------------4:gi|15596160|ref|NP_249654.1| +-10 | | +--5:gi|15794899|ref|NP_284721.1| | +---------------------------------11 +---8 +--6:gi|15676377|ref|NP_273513.1| | | | | +-----------------------1:gi|15602848|ref|NP_245920.1| --7 +----------9 | +-----------------------2:gi|15641179|ref|NP_230811.1| | +-------------------------------------------3:gi|15838454|ref|NP_299142.1|Remember, this is an unrooted tree!
Bootstrapping tree
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|15602848|ref|NP_245920.1| 611 aa Sequence 2: gi|15641179|ref|NP_230811.1| 611 aa Sequence 3: gi|15596160|ref|NP_249654.1| 611 aa Sequence 4: gi|15794899|ref|NP_284721.1| 611 aa Sequence 5: gi|15676377|ref|NP_273513.1| 611 aa Sequence 6: gi|15838454|ref|NP_299142.1| 611 aa Phylogenetic tree file created: [clustal.ph]