CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: gi|15826973|ref|NP_301236.1| 554 aa Sequence 2: gi|15608086|ref|NP_215461.1| 553 aa Sequence 3: gi|15677249|ref|NP_274402.1| 548 aa Sequence 4: gi|15794499|ref|NP_284321.1| 548 aa Sequence 5: gi|15804618|ref|NP_290659.1| 549 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 84.8101 Sequences (1:3) Aligned. Score: 46.7153 Sequences (1:4) Aligned. Score: 46.3504 Sequences (1:5) Aligned. Score: 47.3588 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 46.1679 Sequences (2:4) Aligned. Score: 45.8029 Sequences (2:5) Aligned. Score: 48.0874 Sequences (3:2) Aligned. Score: 46.1679 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 99.2701 Sequences (3:5) Aligned. Score: 56.2044 Sequences (4:2) Aligned. Score: 45.8029 Sequences (4:3) Aligned. Score: 99.2701 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 56.2044 Sequences (5:2) Aligned. Score: 48.0874 Sequences (5:3) Aligned. Score: 56.2044 Sequences (5:4) Aligned. Score: 56.2044 Sequences (5:5) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 4 groups Aligning... Group 1: Sequences: 2 Score:11940 Group 2: Sequences: 3 Score:9445 Group 3: Sequences: 2 Score:11261 Group 4: Sequences: 5 Score:8786 Alignment Score 20429 CLUSTAL-Alignment file created [clustal.aln]Clustal output
CLUSTAL W (1.81) multiple sequence alignment gi|15677249|ref|NP_274402.1| ------MKHLH--DLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQ gi|15794499|ref|NP_284321.1| ------MKHLH--DLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQ gi|15804618|ref|NP_290659.1| ------MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT gi|15826973|ref|NP_301236.1| MTSMQAIPDIT--ATPAWDALRRHHDEIGATHLRQFFADNPNRGRELVIT gi|15608086|ref|NP_215461.1| MTSAP-IPDIT--ATPAWDALRRHHDQIGNTHLRQFFADDPGRGRELTVS : .: .**. * .*.*: : ::* .: * . gi|15677249|ref|NP_274402.1| VG-GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENR gi|15794499|ref|NP_284321.1| VG-GLTLDYSKNRINDETMSLLFELAHEAGVPERMRQMFHGKKINTTENR gi|15804618|ref|NP_290659.1| FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENR gi|15826973|ref|NP_301236.1| VG-DLYIDYSKHRITHDTVQLLVDLARAANLEQRRDQMLAGVHVNTSENR gi|15608086|ref|NP_215461.1| VG-DLYIDYSKHRVTRETLALLIDLARTAHLEERRDQMFAGVHINTSEDR .. : :****:*:. :*: * :**: . : .*: * ::* :*:* gi|15677249|ref|NP_274402.1| AVLHVALRNRTNSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTN gi|15794499|ref|NP_284321.1| AVLHVALRNRTNSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTN gi|15804618|ref|NP_290659.1| AVLHVALRNRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTG gi|15826973|ref|NP_301236.1| SVLHTALRLPRDTELIVDGQNVVQDVHAVLDVMGDFTDRLRSGEWTGATG gi|15608086|ref|NP_215461.1| AVLHTALRLPRDAELVVDGQDVVTDVHAVLDAMGAFTDRLRSGEWTGATG :***.*** :: ::***::*: .*: **: * *:. : **.* * *. gi|15677249|ref|NP_274402.1| QVITDVVNIGIGGSDLGPLMMCTALKPFGHPRLNMHFVSNVDGSQLRDVL gi|15794499|ref|NP_284321.1| QVITDVVNIGIGGSDLGPLMMCTALKPFGHPRLNMHFVSNVDGSQLRDVL gi|15804618|ref|NP_290659.1| KAITDVVNIGIGGSDLGPYMVTEALRPYKN-HLNMHFVSNVDGTHIAEVL gi|15826973|ref|NP_301236.1| KRINTVVNIGIGGSDLGPVMVYQALRHYADAGISARFVSNIDPADLTAKL gi|15608086|ref|NP_215461.1| KRISTVVNIGIGGSDLGPVMVYQALRHYADAGISARFVSNVDPADLIATL : *. ************* *: **: : . :. :****:* :.: * gi|15677249|ref|NP_274402.1| SKVHPETTLFIIASKTFTTQETLTNALTAREWFLNHAGDEEAVAKHFAAV gi|15794499|ref|NP_284321.1| SKVHPETTLFIIASKTFTTQETLTNALTAREWFLNHAGDEEAVAKHFAAV gi|15804618|ref|NP_290659.1| KKVNPETTLFLVASKTFTTQETMTNAHSARDWFLKAAGDEKHVAKHFAAL gi|15826973|ref|NP_301236.1| SDLEPGTTLFIVASKTFSTLETLTNATAARRWLTDALG-EAAVSKHFVAV gi|15608086|ref|NP_215461.1| ADLDPATTLFIVASKTFSTLETLTNATAARRWLTDALG-DAAVSRHFVAV .:.* ****::*****:* **:*** :** *: . * : *::**.*: gi|15677249|ref|NP_274402.1| STNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIGLPIMLYLGEENFIEM gi|15794499|ref|NP_284321.1| STNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIGLPIMLYLGEENFIEM gi|15804618|ref|NP_290659.1| STNAKAVGEFGIDTANMFEFWDWVGGRYSLWSAIGLSIVLSIGFDNFVEL gi|15826973|ref|NP_301236.1| STNKRLVKDFGINTANMFGFWEWVGGRYSVDSAIGLSLMAVVGRESFADF gi|15608086|ref|NP_215461.1| STNKRLVDDFGINTDNMFGFWDWVGGRYSVDSAIGLSLMTVIGRDAFADF *** : * :***:* *** **:*******: *****.:: :* : * :: gi|15677249|ref|NP_274402.1| LNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIAPYDQHLH gi|15794499|ref|NP_284321.1| LNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIAPYDQHLH gi|15804618|ref|NP_290659.1| LSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEAILPYDQYMH gi|15826973|ref|NP_301236.1| LSGFHIVDQHFQNAPLESNAPVLLGLIGLWYSDFLGAQSRAVLPYSNDLA gi|15608086|ref|NP_215461.1| LAGFHIIDRHFATAPLESNAPVLLGLIGLWYSNFFGAQSRTVLPYSNDLS * * * :*:** .:* * * **:*.***:** :: *. :..: **.: : gi|15677249|ref|NP_274402.1| RLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLH gi|15794499|ref|NP_284321.1| RLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLH gi|15804618|ref|NP_290659.1| RFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLIH gi|15826973|ref|NP_301236.1| RFAAYLQQLTMESNGKSTRADGTPVTTNTGEIYWGETGTNGQHAFYQLLH gi|15608086|ref|NP_215461.1| RFPAYLQQLTMESNGKSTRADGSPVSADTGEIFWGEPGTNGQHAFYQLLH *:. ::** ****** . :*. * :*. * ***.* ******:**:* gi|15677249|ref|NP_274402.1| QGTHITPIDLIASLEKRSNLP------GHHEILLANVFAQAEAFMRGKTP gi|15794499|ref|NP_284321.1| QGTHITPIDLIASLEKRSNLP------GHHEILLANVFAQAEAFMRGKTP gi|15804618|ref|NP_290659.1| QGTKMVPCDFIAPAITHNPLS------DHHQKLLSNFFAQTEALAFGKSR gi|15826973|ref|NP_301236.1| QGTRLVPADFIGFSQPIDDLPTVDGIGSMHDLLMSNFFAQTQVLAFGKTA gi|15608086|ref|NP_215461.1| QGTRLVPADFIGFAQPLDDLPTAEGTGSMHDLLMSNFFAQTQVLAFGKTA ***::.* *:*. . *. . *: *::*.***::.: **: gi|15677249|ref|NP_274402.1| DEVRAELKAQGMDEVRIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIA gi|15794499|ref|NP_284321.1| DEVRAELKAQGMDEARIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIA gi|15804618|ref|NP_290659.1| EVVEQEYRDQGKDPATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIA gi|15826973|ref|NP_301236.1| EEIAAEG--------TPAEVVPHKVMPGNRPTTSILANRLTPSVLGQLIA gi|15608086|ref|NP_215461.1| EEIAADG--------TPAHVVAHKVMPGNRPSTSILASRLTPSVLGQLIA : : : :*..*.: ****:. ** .:.* :* *** gi|15677249|ref|NP_274402.1| MYEHKTFVQGIIWGINSFDQWGVELGKQLAKTILGELTGE-TGPQKHDSS gi|15794499|ref|NP_284321.1| MYEHKTFVQGIIWGINSFDQWGVELGKQLAKTILGELTGE-TGSQKHDSS gi|15804618|ref|NP_290659.1| LYEHKIFTQGVILNIFTFDQWGVELGKQLANRILPELKDD-KEISSHDSS gi|15826973|ref|NP_301236.1| LYEHQVFTEGVIWGIDSFDQWGVELGKKQAEALLPVITGNASPAQQLDSS gi|15608086|ref|NP_215461.1| LYEHQVFTEGVVWGIDSFDQWGVELGKTQAKALLPVITGAGSPPPQSDSS :***: *.:*:: .* :********** *: :* :.. . . *** gi|15677249|ref|NP_274402.1| TERLINLYLQTNRK-- gi|15794499|ref|NP_284321.1| TERLINLYLQTNRK-- gi|15804618|ref|NP_290659.1| TNGLINRYKAWRG--- gi|15826973|ref|NP_301236.1| TDTLVRRYRTERGRTS gi|15608086|ref|NP_215461.1| TDGLVRRYRTERGRAG *: *:. * .Tree construction
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|15677249|ref|NP_274402.1| 566 aa Sequence 2: gi|15794499|ref|NP_284321.1| 566 aa Sequence 3: gi|15804618|ref|NP_290659.1| 566 aa Sequence 4: gi|15826973|ref|NP_301236.1| 566 aa Sequence 5: gi|15608086|ref|NP_215461.1| 566 aa Phylogenetic tree file created: [clustal.ph]+--1:gi|15677249|ref|NP_274402.1| +----------------------8 +--------------------7 +--2:gi|15794499|ref|NP_284321.1| | | --6 +-------------------3:gi|15804618|ref|NP_290659.1| | | +------4:gi|15826973|ref|NP_301236.1| +--9 +------5:gi|15608086|ref|NP_215461.1|Midpoint-rooted tree:
+--1:gi|15677249|ref|NP_274402.1| +--------------------------------------8 +----7 +--2:gi|15794499|ref|NP_284321.1| | | --6 +----------------------------------3:gi|15804618|ref|NP_290659.1| | | +------------4:gi|15826973|ref|NP_301236.1| +-------------------------------9 +------------5:gi|15608086|ref|NP_215461.1|Remember, this is an unrooted tree!
Bootstrapping tree
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|15677249|ref|NP_274402.1| 566 aa Sequence 2: gi|15794499|ref|NP_284321.1| 566 aa Sequence 3: gi|15804618|ref|NP_290659.1| 566 aa Sequence 4: gi|15826973|ref|NP_301236.1| 566 aa Sequence 5: gi|15608086|ref|NP_215461.1| 566 aa Phylogenetic tree file created: [clustal.ph]