CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: gi|15804618|ref|NP_290659.1| 549 aa Sequence 2: gi|16131851|ref|NP_418449.1| 549 aa Sequence 3: gi|15602281|ref|NP_245353.1| 549 aa Sequence 4: gi|15677249|ref|NP_274402.1| 548 aa Sequence 5: gi|15794499|ref|NP_284321.1| 548 aa Sequence 6: gi|15599926|ref|NP_253420.1| 554 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 100 Sequences (1:3) Aligned. Score: 76.5027 Sequences (1:4) Aligned. Score: 56.2044 Sequences (1:5) Aligned. Score: 56.2044 Sequences (1:6) Aligned. Score: 47.541 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 76.5027 Sequences (2:4) Aligned. Score: 56.2044 Sequences (2:5) Aligned. Score: 56.2044 Sequences (2:6) Aligned. Score: 47.541 Sequences (3:2) Aligned. Score: 76.5027 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 54.562 Sequences (3:5) Aligned. Score: 54.562 Sequences (3:6) Aligned. Score: 47.3588 Sequences (4:2) Aligned. Score: 56.2044 Sequences (4:3) Aligned. Score: 54.562 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 99.2701 Sequences (4:6) Aligned. Score: 48.5401 Sequences (5:2) Aligned. Score: 56.2044 Sequences (5:3) Aligned. Score: 54.562 Sequences (5:4) Aligned. Score: 99.2701 Sequences (5:5) Aligned. Score: 100 Sequences (5:6) Aligned. Score: 48.5401 Sequences (6:2) Aligned. Score: 47.541 Sequences (6:3) Aligned. Score: 47.3588 Sequences (6:4) Aligned. Score: 48.5401 Sequences (6:5) Aligned. Score: 48.5401 Sequences (6:6) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 5 groups Aligning... Group 1: Sequences: 2 Score:11949 Group 2: Sequences: 3 Score:10749 Group 3: Sequences: 2 Score:11940 Group 4: Sequences: 5 Score:9378 Group 5: Sequences: 6 Score:8983 Alignment Score 32812 CLUSTAL-Alignment file created [clustal.aln]Clustal output
CLUSTAL W (1.81) multiple sequence alignment gi|15804618|ref|NP_290659.1| MKN----INPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSATFD gi|16131851|ref|NP_418449.1| MKN----INPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSATFD gi|15602281|ref|NP_245353.1| MKN----INPTTTNAWKALQQHHKTQSAVTIQQLFAQEKDRFTDYSLSFN gi|15677249|ref|NP_274402.1| MKH----LHDLP--AWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG gi|15794499|ref|NP_284321.1| MKH----LHDLP--AWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG gi|15599926|ref|NP_253420.1| MKHHLTPLDATQLDSWRALAAHRQELQDFRMRQAFIDDPERFKRFSFSAC **: :. :* * * . . . : : * .: :* : gi|15804618|ref|NP_290659.1| DQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAV gi|16131851|ref|NP_418449.1| DQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAV gi|15602281|ref|NP_245353.1| NEVLVDFSKNNVTKETLGLLRQLAQECALSEAVDAMFSGAKINKTEDRAV gi|15677249|ref|NP_274402.1| -GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAV gi|15794499|ref|NP_284321.1| -GLTLDYSKNRINDETMSLLFELAHEAGVPERMRQMFHGKKINTTENRAV gi|15599926|ref|NP_253420.1| -GLFLDFSKNLIRQDTIDLLVKLAEEARLSDAIRAMFDGEAINASERRPV : :*:*** : .:*: * .**.*. :. : ** * ** :* *.* gi|15804618|ref|NP_290659.1| LHVALRNRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKA gi|16131851|ref|NP_418449.1| LHVALRNRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKA gi|15602281|ref|NP_245353.1| LHTALRNRSNSPVLVDGKDVMPEVNAVLAKMKDFCHRVISGEWKGYTGKA gi|15677249|ref|NP_274402.1| LHVALRNRTNSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQV gi|15794499|ref|NP_284321.1| LHVALRNRTNSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQV gi|15599926|ref|NP_253420.1| LHTALRRPIGDKVLVDGVDVMPEVHRVLHQMTELVGYVHNGLWRGYTEKP **.***. . ::*** ****:*: ** :* : : .* * *** : gi|15804618|ref|NP_290659.1| ITDVVNIGIGGSDLGPYMVTEALRPYKN-HLNMHFVSNVDGTHIAEVLKK gi|16131851|ref|NP_418449.1| ITDVVNIGIGGSDLGPYMVTEALRPYKN-HLNMHFVSNVDGTHIAEVLKK gi|15602281|ref|NP_245353.1| ITDVVNIGIGGSDLGPYMVTEALRPYKN-HLNLHFVSNVDGTHIAETLKK gi|15677249|ref|NP_274402.1| ITDVVNIGIGGSDLGPLMMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSK gi|15794499|ref|NP_284321.1| ITDVVNIGIGGSDLGPLMMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSK gi|15599926|ref|NP_253420.1| ITDVVNIGIGGSFLGPQLVSEALLPFAQKGVRCHYLANIDGSEFHELASR ************ *** :: ** *: : :. *:::*:**:.: : .: gi|15804618|ref|NP_290659.1| VNPETTLFLVASKTFTTQETMTNAHSARDWFLKAAGDEKHVAKHFAALST gi|16131851|ref|NP_418449.1| VNPETTLFLVASKTFTTQETMTNAHSARDWFLKAAGDEKHVAKHFAALST gi|15602281|ref|NP_245353.1| VNPETTLFLVASKTFTTQETMTNAHSARNWFLATAKDESHVAKHFAALST gi|15677249|ref|NP_274402.1| VHPETTLFIIASKTFTTQETLTNALTAREWFLNHAGDEEAVAKHFAAVST gi|15794499|ref|NP_284321.1| VHPETTLFIIASKTFTTQETLTNALTAREWFLNHAGDEEAVAKHFAAVST gi|15599926|ref|NP_253420.1| LNAETTLFIVSSKSFGTLETLKNAQAARAWYLAQGGTEEELYRHFIAVSS ::.*****:::**:* * **:.** :** *:* . *. : :** *:*: gi|15804618|ref|NP_290659.1| NAKAVGEFGIDTANMFEFWDWVGGRYSLWSAIGLSIVLSIGFDNFVELLS gi|16131851|ref|NP_418449.1| NAKAVGEFGIDTANMFEFWDWVGGRYSLWSAIGLSIVLSIGFDNFVELLS gi|15602281|ref|NP_245353.1| NSKAVAEFGIDTNNMFEFWDWVGGRYSLWSAIGLSIALSIGFEHFEALLA gi|15677249|ref|NP_274402.1| NQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIGLPIMLYLGEENFIEMLN gi|15794499|ref|NP_284321.1| NQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIGLPIMLYLGEENFIEMLN gi|15599926|ref|NP_253420.1| NKEAAIAFGIREENIFPMWDWVGGRYSLWSAIGLPIAMSIGISNFKELLS * :*. *** *:* :****************.* : :* .:* :* gi|15804618|ref|NP_290659.1| GAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEAILPYDQYMHRF gi|16131851|ref|NP_418449.1| GAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEAILPYDQYMHRF gi|15602281|ref|NP_245353.1| GAHEMDKHFRTAPIEQNIPTTLALIGLWNTNFLGAQTEAILPYDQYLHRF gi|15677249|ref|NP_274402.1| GAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIAPYDQHLHRL gi|15794499|ref|NP_284321.1| GAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIAPYDQHLHRL gi|15599926|ref|NP_253420.1| GAYNMDQHFQTAPFERNIPVLLGLLGVWYGDFWGANSHAILPYDYYLRNI **: **:** .:* *:*:*. *.*:*:* :: *. :..* *** :::.: gi|15804618|ref|NP_290659.1| AAYFQQGNMESNGKYVDRNGNVVD-YQTGPIIWGEPGTNGQHAFYQLIHQ gi|16131851|ref|NP_418449.1| AAYFQQGNMESNGKYVDRNGNVVD-YQTGPIIWGEPGTNGQHAFYQLIHQ gi|15602281|ref|NP_245353.1| AAYFQQGNMESNGKYVDRNGEVIDNYQTGPIIWGEPGTNGQHAFYQLIHQ gi|15677249|ref|NP_274402.1| PKFIQQLDMESNGKQVTLDGKAVG-HETSPIIWGETGINGQHAFFQLLHQ gi|15794499|ref|NP_284321.1| PKFIQQLDMESNGKQVTLDGKAVG-HETSPIIWGETGINGQHAFFQLLHQ gi|15599926|ref|NP_253420.1| TDHLQQLDMESNGKSVRQDGTPVT-SGTGPVIWGGVGCNGQHAYHQLLHQ . .:** :****** * :* : *.*:*** * *****:.**:** gi|15804618|ref|NP_290659.1| GTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEY gi|16131851|ref|NP_418449.1| GTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEY gi|15602281|ref|NP_245353.1| GTTLIPCDFIAPAQTHNPLADHHEKLLSNFFAQTEALAFGKTKEEVEAEF gi|15677249|ref|NP_274402.1| GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAEL gi|15794499|ref|NP_284321.1| GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAEL gi|15599926|ref|NP_253420.1| GTQLIPADFIVPVSSYNPVADHHQWLYANCLSQSQALMLGKSREEAEAEL ** : * *:*.. . . :..**: * :* ::*::*: **: : .. * gi|15804618|ref|NP_290659.1| RDQGKDPATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIF gi|16131851|ref|NP_418449.1| RDQGKDPATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIF gi|15602281|ref|NP_245353.1| VKAGKSLDEVKEVVPFKVFTGNKPTNSILVQKITPFTLGALIAMYEHKIF gi|15677249|ref|NP_274402.1| KAQGMDEVRIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTF gi|15794499|ref|NP_284321.1| KAQGMDEARIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTF gi|15599926|ref|NP_253420.1| RAKGLPEAEVQRLAPHKVIPGNRPSNTLVVERISARRLGALIAMYEHKVY * :. :.*.*.: **:*:* ::: .:.. :*:***:**** : gi|15804618|ref|NP_290659.1| TQGVILNIFTFDQWGVELGKQLANRILPELKDDKEISSHDSSTNGLINRY gi|16131851|ref|NP_418449.1| TQGVILNIFTFDQWGVELGKQLANRILPELKDDKEISSHDSSTNGLINRY gi|15602281|ref|NP_245353.1| VQGVMFNIYSFDQWGVELGKQLANRILPELANRETITTHDSSTNGLINQY gi|15677249|ref|NP_274402.1| VQGIIWGINSFDQWGVELGKQLAKTILGELTGETGPQKHDSSTERLINLY gi|15794499|ref|NP_284321.1| VQGIIWGINSFDQWGVELGKQLAKTILGELTGETGSQKHDSSTERLINLY gi|15599926|ref|NP_253420.1| VQSILWGINAFDQWGVELGKELGKGVYSRLVGSEETPAEDASTQGLIDFF .*.:: .* :**********:*.: : .* . .*:**: **: : gi|15804618|ref|NP_290659.1| KAWRG- gi|16131851|ref|NP_418449.1| KAWRG- gi|15602281|ref|NP_245353.1| KQWR-- gi|15677249|ref|NP_274402.1| LQTNRK gi|15794499|ref|NP_284321.1| LQTNRK gi|15599926|ref|NP_253420.1| RGRHRG .Tree construction
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|15804618|ref|NP_290659.1| 556 aa Sequence 2: gi|16131851|ref|NP_418449.1| 556 aa Sequence 3: gi|15602281|ref|NP_245353.1| 556 aa Sequence 4: gi|15677249|ref|NP_274402.1| 556 aa Sequence 5: gi|15794499|ref|NP_284321.1| 556 aa Sequence 6: gi|15599926|ref|NP_253420.1| 556 aa Phylogenetic tree file created: [clustal.ph]+--1:gi|15804618|ref|NP_290659.1| +----------8 | +--2:gi|16131851|ref|NP_418449.1| --7 | +-----------3:gi|15602281|ref|NP_245353.1| | | +--9 +--4:gi|15677249|ref|NP_274402.1| | +-------------------11 +--------10 +--5:gi|15794499|ref|NP_284321.1| | +-----------------------------6:gi|15599926|ref|NP_253420.1|Midpoint-rooted tree:
+--1:gi|15804618|ref|NP_290659.1| +-------------------8 +----------------9 +--2:gi|16131851|ref|NP_418449.1| | | +-----10 +-------------------3:gi|15602281|ref|NP_245353.1| | | | | +--4:gi|15677249|ref|NP_274402.1| --7 +-----------------------------------11 | +--5:gi|15794499|ref|NP_284321.1| | +-------------------------------------------6:gi|15599926|ref|NP_253420.1|Remember, this is an unrooted tree!
Bootstrapping tree
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|15804618|ref|NP_290659.1| 556 aa Sequence 2: gi|16131851|ref|NP_418449.1| 556 aa Sequence 3: gi|15602281|ref|NP_245353.1| 556 aa Sequence 4: gi|15677249|ref|NP_274402.1| 556 aa Sequence 5: gi|15794499|ref|NP_284321.1| 556 aa Sequence 6: gi|15599926|ref|NP_253420.1| 556 aa Phylogenetic tree file created: [clustal.ph]