CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: gi|15678615|ref|NP_275730.1| 651 aa Sequence 2: gi|14590837|ref|NP_142909.1| 723 aa Sequence 3: gi|15669449|ref|NP_248259.1| 651 aa Sequence 4: gi|16082174|ref|NP_394618.1| 547 aa Sequence 5: gi|13542051|ref|NP_111739.1| 543 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 43.318 Sequences (1:3) Aligned. Score: 41.321 Sequences (1:4) Aligned. Score: 35.4662 Sequences (1:5) Aligned. Score: 35.175 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 38.5561 Sequences (2:4) Aligned. Score: 41.4991 Sequences (2:5) Aligned. Score: 40.3315 Sequences (3:2) Aligned. Score: 38.5561 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 31.6271 Sequences (3:5) Aligned. Score: 32.7808 Sequences (4:2) Aligned. Score: 41.4991 Sequences (4:3) Aligned. Score: 31.6271 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 72.3757 Sequences (5:2) Aligned. Score: 40.3315 Sequences (5:3) Aligned. Score: 32.7808 Sequences (5:4) Aligned. Score: 72.3757 Sequences (5:5) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 4 groups Aligning... Group 1: Sequences: 2 Score:10488 Group 2: Sequences: 3 Score:8482 Group 3: Sequences: 2 Score:10198 Group 4: Sequences: 5 Score:8280 Alignment Score 16365 CLUSTAL-Alignment file created [clustal.aln]Clustal output
CLUSTAL W (1.81) multiple sequence alignment gi|16082174|ref|NP_394618.1| MVQIKILVNCALPYANGPLHIGHIAGAYLGADVFVRYNRLMGNQVLYVSG gi|13542051|ref|NP_111739.1| MIGIKILVNCALPYANGPLHVGHIAGAYLGADVFVRFNRLIGNEVLYVSG gi|14590837|ref|NP_142909.1| --MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEDVVFICG gi|15678615|ref|NP_275730.1| --MSKVFITCALPYANGPTHLGHLRSTYIPADIYARYRRLRGDDVLFVCA gi|15669449|ref|NP_248259.1| ---MRYLITTALAYTNGPLHLGHARSTYIPADIIYKYLKLRGEDVIHVGG : ::. **.*:*** * ** .:*: **: :: :* *::*:.: . gi|16082174|ref|NP_394618.1| SDEYGTPITVRAEKEGRSPKEIADIYYEEHLRTFENLGISFDIFMRTTWP gi|13542051|ref|NP_111739.1| SDEYGTPITVRAEKEGKSPQEIADRYYAEHIETFKKLGINFDIFMRTTYP gi|14590837|ref|NP_142909.1| TDEHGTPISFRALKEGRSPREIVDEFHEHIKITFQRAKISFDFFGRTELP gi|15678615|ref|NP_275730.1| TDEHGTPIAVKAEAEGRTPLEVATRYHEMIRGDLERCDISFDSFTRTTDE gi|15669449|ref|NP_248259.1| TDNHGVPITLTAEKEGKSPEEIVEKYHNEIKEDLDLLGVEFDAFGKTHSQ :*::*.**:. * **::* *:. :: :. :.** * :* gi|16082174|ref|NP_394618.1| EHSENAQDFFIKLLNEGYIEKGTMIAPFCRKIGRFMPDRYIEGTCPYCHY gi|13542051|ref|NP_111739.1| EHSSVAQEFFLKLLNEGVIEKGTMIAPYCRKIGRFMPDRYIEGECPYCGY gi|14590837|ref|NP_142909.1| IHYKLSQQFFLKAYENGHLVKKVTKQAYCEHDKMFLPDRFVIGTCPFCGA gi|15678615|ref|NP_275730.1| LHHEIAQKFFLKLYEKGFIYEKDIKQLYCGDCQRFLPDRYVEGTCPYCGS gi|15669449|ref|NP_248259.1| IHIETAQEFYLKLKENGYIYEKEIEQFYCPNCKKFLPDRYVEGICPYCGG * . :*.*::* ::* : : :* . *:***:: * **:* gi|16082174|ref|NP_394618.1| PKARGDQCDNCGRTLDPQDLIDPKCILSGETPEFRETEHFFLRLDLLEDR gi|13542051|ref|NP_111739.1| KKARGDQCDNCGRTLDPQELINPVCILSGETPEFRETEHFFLRLDLFEER gi|14590837|ref|NP_142909.1| ENQRGDQCEVCGRPLTPEILINPRCALCGRPISFRESAHYYIKMQDFAEK gi|15678615|ref|NP_275730.1| EGARGDHCEGCGRHLEPLQLEDPRCMVCGSEPEVRDSRHYFFRLNQFQDE gi|15669449|ref|NP_248259.1| E-ARGDHCEVCGRHLEPFELKDPYCVICKGKPEIRKTKHHFFKLSALKKE ***:*: *** * * * :* * :. ..*.: *.::::. : .. gi|16082174|ref|NP_394618.1| LKSWISSKNFWKPNVLAYTQNFISGGLKKRPITRDIDWGVKIPLDG--YD gi|13542051|ref|NP_111739.1| LEEWLKTKTFWKPNVLAYTRNFIQGGLKKRAITRDIDWGVKVPLEG--YE gi|14590837|ref|NP_142909.1| LKRWIENQ-PWKPNVKNMVLKWIEEGLEERAITRDLNWGIPVPLDEEDMK gi|15678615|ref|NP_275730.1| IREWIE-GSEMPSNVRNYAIQWLREGLKDWILTRDMEWGVQVPLEG--NE gi|15669449|ref|NP_248259.1| LEEYIKNAKEMPEHVKNMALNWIKE-LHDWDISRDISWGVPIPG-----T :. ::. :* . ::: *.. ::**:.**: :* gi|16082174|ref|NP_394618.1| SKRIYVWFEALIGYITGAKEYSKRTGNPDLWKEYYM--DPEVRNYYFIGK gi|13542051|ref|NP_111739.1| HKRIYVWFEALIGYITGAKMFSEVIGKPNYWKEFYF--DKDVKNYYFIGK gi|14590837|ref|NP_142909.1| NKVLYVWFEAPIGYISITMEYFKRLGKPNEWKKYWLNIDSETRVIHFIGK gi|15678615|ref|NP_275730.1| GKIIYVWGEAFLGYISSAAAWSKKTG--RDWREYW-----DSGAIHFIGK gi|15669449|ref|NP_248259.1| NQVMYVWLEAPIGYIS----FTKMLG--EIWKKYWL--EKDTKIYHFIGK : :*** ** :***: : : * *:::: : :**** gi|16082174|ref|NP_394618.1| DNIPFHAIIWPAMLMGYG---------GFNLPYDIPANEYLTFKGQQFSK gi|13542051|ref|NP_111739.1| DNIPFHSIIWPAMLLGYG---------ELNLPYDIPANEYLTFKGEQFSK gi|14590837|ref|NP_142909.1| DNIPFHAIFWPAFLMAYGKYKDEEVEAEWNLPYDIPANEYLTLEGKKFST gi|15678615|ref|NP_275730.1| DIIYHHAIFWPALLMAYG----------CRTPANIIAGEYLSLEGQKMST gi|15669449|ref|NP_248259.1| DITVHHAVFWPGMLIAHGS---------FNLPTAVVSGGYLTLEGRKMST * .*:::**.:*:.:* . * : :. **:::*.::*. gi|16082174|ref|NP_394618.1| SRGIGYSVDDLLKAVPADYLRYYVASILPETGDSDFSLEELVNTVNSDLI gi|13542051|ref|NP_111739.1| SRGIGYTVDELLRVIPPDYLRYYVASILPETGDSDFSLEELVKTVNSDLI gi|14590837|ref|NP_142909.1| SRNWAIWVHEFLDVFPADYLRYYLTTIMPETRDSDFSFAEFKTRINEELV gi|15678615|ref|NP_275730.1| SKNWVVWTSDFLERFDRDLLRYYLTVNAPLTRDTDFSWDDFQRRVNDELA gi|15669449|ref|NP_248259.1| SKRWVVWVKDFVKNFDADYLRYYLIMSAPLFKDCDFSFDDFKNKINNELI *: . ::: . * ****: * * *** :: :*.:* gi|16082174|ref|NP_394618.1| DKYGNLVYRILSFMDRYSINPDP------VDVSGDEEFKYAQRSFELWSE gi|13542051|ref|NP_111739.1| DKYGNFVHRTLSFINKYDLKITK------PNSLNDEAFEYAQNAFKEYCD gi|14590837|ref|NP_142909.1| NNLGNFVHRAMTFVNRYFDGIVPERG--ELDELDRQALEEIEKAFEEVGE gi|15678615|ref|NP_275730.1| DVLGNFLHRTFSFTGRFFDGRVPAAG--ELTAEDLEFLESIRAAHDTVAE gi|15669449|ref|NP_248259.1| NIIGNFTHRVLTFTHRKFK-KVPIVDEDRLKEEDKELLKKCEETLEAVDK : **: :* ::* : . : :: . : . . gi|16082174|ref|NP_394618.1| DLSGVHVKRGLLRWLDLAIYANGFFNRSEPWKTIKNDRQKLNHDLYLSLK gi|13542051|ref|NP_111739.1| ELSQVHIKRSLAIWLNLAIYANSYFNKSEPWNLIKTDRDQCNYKLYVSLK gi|14590837|ref|NP_142909.1| LIMNYRFKDALKRVMELASFGNKYFDHKQPWKTAKEDRARTGTTVNISLQ gi|15678615|ref|NP_275730.1| LLDKFQFRDALMHIIKLAKRGNKYFNDQEPWKAVKESPARASTCLHLCNL gi|15669449|ref|NP_248259.1| NIRSFKFRDALVNILHLAIEGNSYFQKMEPWAVDDE--ERLKEILYTCCK : :.: .* :.** .* :*: :** . : : . gi|16082174|ref|NP_394618.1| ITEVLTVMVYPYIPSSAEKIWNMLGIPFAIGQSYRHLYEMHAFSVHRGAI gi|13542051|ref|NP_111739.1| LAQYLTAMIYPFTPTSAKAIWEQLGVNMDIDSSFSILNSINDFSVKPSRI gi|14590837|ref|NP_142909.1| IVKALGILLEPFLPDASEKIWHLLNLDEVKKWKFKELPAG--HRVRKAEI gi|15678615|ref|NP_275730.1| LAANLGKLLRPFMPSAAGRILSIMNLEDEA---WGFHELREGQVIERAKP gi|15669449|ref|NP_248259.1| TVKTLVYLLYPYMPKKSLALLELMNEELD-------LELR-GNELKKPKI . * :: *: * : : :. :. gi|16082174|ref|NP_394618.1| PFEKIDLESLVSKLNN---------------------------------- gi|13542051|ref|NP_111739.1| PFEKLDIDKLKL-------------------------------------- gi|14590837|ref|NP_142909.1| LFKKVTDEQIIYFILNYMGRNNPEGAKMLLEKYYKREDVIKVAKEKFGEE gi|15678615|ref|NP_275730.1| LFSKITDEAIEEEKAKLIEEDDE--------------------------- gi|15669449|ref|NP_248259.1| IFKKIDNKKIEEMKKKLYENKKEET------------------------- *.*: . : gi|16082174|ref|NP_394618.1| -------------------------------------------------- gi|13542051|ref|NP_111739.1| -------------------------------------------------- gi|14590837|ref|NP_142909.1| SKIILKRIYKDIKLEEGKEEKEMEYVKFEDFAKLDLRVGKIIEVKDHPNA gi|15678615|ref|NP_275730.1| ----------------------VETVTIDDFTTMDIRVGVIRSAERIEGS gi|15669449|ref|NP_248259.1| -----------------KGGEKMEQIDISYLEKIDLRVGEVVEAEDIPKS gi|16082174|ref|NP_394618.1| -------------------------------------------------- gi|13542051|ref|NP_111739.1| -------------------------------------------------- gi|14590837|ref|NP_142909.1| DKLYIVKVDLGKEVRTLVAGLKKYYKPEELLNKYVIIVANLEPKKLRGVE gi|15678615|ref|NP_275730.1| DKLLKLIIDVGEREMQVVAGLAEKYSPEDLVERKITVLVNLKPAKLFGVK gi|15669449|ref|NP_248259.1| KKLLKLMVDLGDEKRQIVSGIKGYYKPEDLVGKKVIVICNLKPAKLCGVL gi|16082174|ref|NP_394618.1| ------------------------------- gi|13542051|ref|NP_111739.1| ------------------------------- gi|14590837|ref|NP_142909.1| SQGMLLAADDGE-NVALLMPDKEVKLGAKVR gi|15678615|ref|NP_275730.1| SEGMVLATGES---LNILDPG-DAEVGERIR gi|15669449|ref|NP_248259.1| SEGMILAAEDDEGNVSLLTVDKDIKAGSKVRTree construction
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|16082174|ref|NP_394618.1| 731 aa Sequence 2: gi|13542051|ref|NP_111739.1| 731 aa Sequence 3: gi|14590837|ref|NP_142909.1| 731 aa Sequence 4: gi|15678615|ref|NP_275730.1| 731 aa Sequence 5: gi|15669449|ref|NP_248259.1| 731 aa Phylogenetic tree file created: [clustal.ph]+-----------------1:gi|16082174|ref|NP_394618.1| +----------------------8 +-----7 +-----------------2:gi|13542051|ref|NP_111739.1| | | --6 +------------------------------3:gi|14590837|ref|NP_142909.1| | | +-------------------------------4:gi|15678615|ref|NP_275730.1| +--9 +--------------------------------5:gi|15669449|ref|NP_248259.1|Midpoint-rooted tree:
+------------------1:gi|16082174|ref|NP_394618.1| +------------------------8 | +-------------------2:gi|13542051|ref|NP_111739.1| --6 | +----------------------------------3:gi|14590837|ref|NP_142909.1| +--7 | +------------------------------------4:gi|15678615|ref|NP_275730.1| +------9 +------------------------------------5:gi|15669449|ref|NP_248259.1|Remember, this is an unrooted tree!
Bootstrapping tree
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|16082174|ref|NP_394618.1| 731 aa Sequence 2: gi|13542051|ref|NP_111739.1| 731 aa Sequence 3: gi|14590837|ref|NP_142909.1| 731 aa Sequence 4: gi|15678615|ref|NP_275730.1| 731 aa Sequence 5: gi|15669449|ref|NP_248259.1| 731 aa Phylogenetic tree file created: [clustal.ph]