CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: gi|14601141|ref|NP_147669.1| 959 aa Sequence 2: gi|14590812|ref|NP_142884.1| 967 aa Sequence 3: gi|14521079|ref|NP_126554.1| 967 aa Sequence 4: gi|11499997|ref|NP_071243.1| 932 aa Sequence 5: gi|15668814|ref|NP_247617.1| 942 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 39.1032 Sequences (1:3) Aligned. Score: 39.1032 Sequences (1:4) Aligned. Score: 34.6567 Sequences (1:5) Aligned. Score: 34.3949 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 91.2099 Sequences (2:4) Aligned. Score: 40.2361 Sequences (2:5) Aligned. Score: 49.0446 Sequences (3:2) Aligned. Score: 91.2099 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 39.8069 Sequences (3:5) Aligned. Score: 48.5138 Sequences (4:2) Aligned. Score: 40.2361 Sequences (4:3) Aligned. Score: 39.8069 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 45.4936 Sequences (5:2) Aligned. Score: 49.0446 Sequences (5:3) Aligned. Score: 48.5138 Sequences (5:4) Aligned. Score: 45.4936 Sequences (5:5) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 4 groups Aligning... Group 1: Sequences: 2 Score:15008 Group 2: Sequences: 2 Score:20466 Group 3: Sequences: 4 Score:15111 Group 4: Sequences: 5 Score:14008 Alignment Score 30411 CLUSTAL-Alignment file created [clustal.aln]Clustal output
CLUSTAL W (1.81) multiple sequence alignment gi|11499997|ref|NP_071243.1| ---MSDFRIIEEKWQKAWEKDRIFESDPNEK---EKFFLTIPYPYLNGNL gi|15668814|ref|NP_247617.1| MMVMIDFKEIEKKWQKRWEEAKIFEANPDDR---EKFFITAAFPYLNGVL gi|14590812|ref|NP_142884.1| -MAELNFKAIEEKWQKRWLEAKIFEPNIRDKPKEKKFYITVAFPYLSGHL gi|14521079|ref|NP_126554.1| -MPELNFRAIEEKWQKRWLEAKVFEPNIKDKPKEKKFYITVAFPYLSGHL gi|14601141|ref|NP_147669.1| ---MERLKAVEEKWQERWREARLFEADPQPG--RRKFFITFPYPYVNAYP :: :*:***: * : ::**.: .**::* .:**:.. gi|11499997|ref|NP_071243.1| HAGHTRTFTIGDAFARYMRMKGYNVLFPLGFHVTGTPIIGLAELIAKRDE gi|15668814|ref|NP_247617.1| HAGHLRTFTIPEVVARFQRMKNKNVLWTFGYHVTGTPILGLAELIKNRDE gi|14590812|ref|NP_142884.1| HVGHARTYTIPDVIARFKRMQGYNVLFPMAWHITGSPIVGIAERIKNRDP gi|14521079|ref|NP_126554.1| HVGHARTYTIPDVIARFKRMQGYNVLFPMGWHITGSPIVGIAERIKNRDP gi|14601141|ref|NP_147669.1| HLGSAFTILRVDIMARYKRMRGYNVLFPQGWHATGGPIVSSALRVREGDP * * * : .**: **:. ***:. .:* ** **:. * : : * gi|11499997|ref|NP_071243.1| RTIEVYTKYHDVPLEDLLQLTTPEKIVEYFSREALQALKSIGYSIDWRRV gi|15668814|ref|NP_247617.1| KTIWAYTELHGIPKEELLELTTPEKIVEYFSKKAEEAFKRMGFSLDWRRN gi|14590812|ref|NP_142884.1| KTIWIYRDVYKVPEEILWTFEDPINIVKYFMKAAKETFIRAGFSVDWSRE gi|14521079|ref|NP_126554.1| HTIWIYRDVYKVPEEILWTFEDPVNIVKYFMKAAKETFIRAGFSVDWSRE gi|14601141|ref|NP_147669.1| RIIKTLRDMG-IPEEDIPRFRDPRYWVEFFTKAWRRDLERYGMSIDWRRE : * . :* * : : * *::* : . : * *:** * gi|11499997|ref|NP_071243.1| FTTTD--EEYQRFIEWQYWKLKELGLIVKGTHPVRYCPHDQNPVEDHDLL gi|15668814|ref|NP_247617.1| FKTDD--KVFNKFIEWQFHKLKEKGLIVKGSHPVRYCPRCDNPVEDHDIL gi|14590812|ref|NP_142884.1| FYTTSLFPPFSKFIEWQFWKLKEKGYIVKGAHRVRWDPVVGTPLGDHDLM gi|14521079|ref|NP_126554.1| FYTTSLFPPFSKFIEWQFLKLKEKGYIVKGAHRVRWDPVVGTPLGDHDLM gi|14601141|ref|NP_147669.1| FYTTSLNPAYSRFIEWQYLKLREKGFVGKGRHPVVWCPKEQKVVGDHDRP * * . :.:*****: **:* * : ** * * : * . : *** gi|11499997|ref|NP_071243.1| AGEE-ATIVEFTVIKFRLEDGD--LIFPCATLRPETVFGVTNIWVKP-TT gi|15668814|ref|NP_247617.1| VGEN-ATLVEYILIKFTTEDG---CIMPMATLRPETVFGVTNVWVNPEAT gi|14590812|ref|NP_142884.1| EGED-VPILDYIIIKFELRENGEVIYLPAATLRPETVYGVTNMWVNPNAT gi|14521079|ref|NP_126554.1| EGED-VPILEYVIIKFELKEGDETIYLPAATLRPETVYGVTNMWINPNAT gi|14601141|ref|NP_147669.1| DEYAGISPQEAVIIKFRGRDG---LVYPALTYRPETVFGVTNLWVHPDAT . : :*** .:. * * *****:****:*::* :* gi|11499997|ref|NP_071243.1| YVIAEVDGEK-------------WFVSKEAYEKLTYTEKKVRLLEEVDAS gi|15668814|ref|NP_247617.1| YVKAKVYLEKETENGIELIENGIWIMAKECAEKLKHQDRKIEIIEEFKGE gi|14590812|ref|NP_142884.1| YVKAKVRRKDKEE---------TWIVSKEAAYKLSFQDREIEVIEEFKGE gi|14521079|ref|NP_126554.1| YVKAKVKRGGKEE---------TWIISKEAAYKLSFQDREIEVIEEFKGE gi|14601141|ref|NP_147669.1| YLVAEVDGEER------------WIIGEQAARELADQGHRVVILERIEGR *: *:* *::.::. :* :.: ::*.... gi|11499997|ref|NP_071243.1| QFFGKYVIVPLVNRKVPILPAEFVDTDNATGVVMSVPAHAPFDLAAIEDL gi|15668814|ref|NP_247617.1| QLINKKVKNPVTGKEVPILPAKFVKTNIGTGCVMSVPAHAPYDYIALRDL gi|14590812|ref|NP_142884.1| KLIGKYVRNPVSGDEVIILPAEFVDPDNATGVVMSVPAHAPFDHVALEDL gi|14521079|ref|NP_126554.1| KLIGKYVRNPVTGDEVIILPAEFVDPDNATGVVMSVPAHAPFDHVALEDL gi|14601141|ref|NP_147669.1| RLLGRIVVNPADGREVPVLPASFVRPDLGTGVVMSVPAHAPYDYVALMEL :::.: * * . :* :***.** .: .** *********:* *: :* gi|11499997|ref|NP_071243.1| KRDEETLAKYGIDKSVVESIKPIVLIKTDIEG-VPAEKLIRELGVKSQKD gi|15668814|ref|NP_247617.1| G--------------LVDEIGLIPLINVPGYGKYPAKEIVEKMGIKSQEE gi|14590812|ref|NP_142884.1| KRETEILEKYDIDPRIVENITYISLIKLEGYGDFPAVEEVNKLGIKSQKD gi|14521079|ref|NP_126554.1| KRESEILVKYDIDPRIVEEITYISLIKLEGYGEFPAVEEVQKLGIKSQKD gi|14601141|ref|NP_147669.1| KRRPETLREYGLEPGVVESLEPIQLIAVPRAEGLLVVEEVRRRGVESQMD :*:.: * ** . : :.. *::** : gi|11499997|ref|NP_071243.1| KELLDKATKTLYKKEYHTGIMLDNTMNYAGMKVSEAKERVHEDLVKLGLG gi|15668814|ref|NP_247617.1| EDKLEEATKKIYKDEFHKGVLNENCLDYEGIPVREIKDKLTKDLIDKGLA gi|14590812|ref|NP_142884.1| KEKLEQATKTIYKAEYHKGIFKVP--PYEGKPVQEVKEAIAKEMLEKGIA gi|14521079|ref|NP_126554.1| REKLEQATKTIYKAEYHKGIFKVP--PYDGKPVQEVKELIAKEMMEKGIA gi|14601141|ref|NP_147669.1| REKLDEATREVYAREFYEGIMLETTGRFSGLKVAEAKEKVVEWLEERGAA .: *::**: :* *:: *:: : * * * *: : : : . * . gi|11499997|ref|NP_071243.1| DVFYEFSEKPVICRCGTKCVVKVVRDQWFLNYSNREWKEKVLNHLEKMRI gi|15668814|ref|NP_247617.1| EIMYEFSEEKVICRCGTPCIVKMVKGQWFIKYSDEKWKELAHKCIDKMRF gi|14590812|ref|NP_142884.1| EIMYEFAEKNVISRFGNRAVIKIIHDQWFIDYGNPEWKEKARKALERMKI gi|14521079|ref|NP_126554.1| EIMYEFAEKNVISRFGNRAVIKIIHDQWFIDYGNSEWKEKARKALARMKI gi|14601141|ref|NP_147669.1| LRIYTLP-QEVYCRCGARTHVKIVEDQWFLLYSKPEWKALAREAVARMEF :* :. : * .* * :*::..***: *.. :** . : : :*.: gi|11499997|ref|NP_071243.1| IPDYYKEEFRNKIEWLRDKACARRKGLGTRIPWDKEWLIESLSDSTIYMA gi|15668814|ref|NP_247617.1| IPENLRQVFHEKIDWMKDKACVRRRGLGTKFPFEEGWVIESLSDSTIYPA gi|14590812|ref|NP_142884.1| LPETRRAQFEAIIDWLDKKACARKIGLGTPLPWDPEWVIESLSDSTIYMA gi|14521079|ref|NP_126554.1| YPETRRAQFEAIIDWLDKKACARKVGLGTPLPWDPEWVIESLSDSTIYMA gi|14601141|ref|NP_147669.1| LPGHVRRDFEAIIEALRDWAFTHKGELGTPLPWDREWVIESLSDSTIYMA * : *. *: : . * .:: *** :*:: *:********** * gi|11499997|ref|NP_071243.1| YYILAKYIN----AGLLKAENMTPEFLDYVLLGKGEVG---KVAEASKLS gi|15668814|ref|NP_247617.1| YYTVAKYIN----QHNIKPEQLTLELFDYVFLGKGDVD---KIAKETGIP gi|14590812|ref|NP_142884.1| YYTISRHINKLRQEGKLDPEKLTPEFFDYIFLEEFSEDKEKELEKKTGIP gi|14521079|ref|NP_126554.1| YYTISRHINRLREEGRLDPEKLTPEFFDYIFLEEFSEEREKELEKKTGIP gi|14601141|ref|NP_147669.1| YYTIAKYTQHP-EKYGVEPEMLTPEVFDYVFLGAGDPG---EVSRRSGIP ** :::: : :..* :* *.:**::* . :: . : :. gi|11499997|ref|NP_071243.1| VELIQQIRDDFEYWYPVDLRSSGKDLVANHLLFYLFHHVAIFPPDKWPRA gi|15668814|ref|NP_247617.1| KDIIEGMRKEFIYYYPVDWRCSAKDLIPNHLTFYIFNHVAIFPEEFWPRG gi|14590812|ref|NP_142884.1| AEIIHEMKEEFEYWYPLDWRCSGKDLIPNHLTFFIFNHVAIFREEHWPKG gi|14521079|ref|NP_126554.1| AEIIHEMKEEFEYWYPLDWRCSGKDLIPNHLTFFIFNHVAIFREEHWPKG gi|14601141|ref|NP_147669.1| QGLLEEMRREFLYWYPLDMRISGKDLIPNHLVFFIFHHTAIFPRELWPRA ::. :: :* *:**:* * *.***:.*** *::*:*.*** : **:. gi|11499997|ref|NP_071243.1| IAVNGYVSLEGKKMSKSKGPLLTMKRAVQQYGADVTRLYILHAAEYD--S gi|15668814|ref|NP_247617.1| IVVNGYVTIEGKKLSKSKGPVLPVLEVAEKFGADVGRFYITTCAELP--Q gi|14590812|ref|NP_142884.1| IAVNGFGTLEGQKMSKSKGNVLNFIDAIEENGADVVRLYIMSLAEHD--S gi|14521079|ref|NP_126554.1| IAVNGFGTLEGQKMSKSKGNVLNFIDAIEENGADVVRLYIMSLAEHD--S gi|14601141|ref|NP_147669.1| IGVNGWVLVAGEKMSKSKGNFILLRQALDWWGADATRWAEVLAGADSGLD * ***: : *:*:***** .: . . : ***. * . . gi|11499997|ref|NP_071243.1| DADWKSREVEGLANHLRRFYNLVKENYLK--EVG-ELTTLDRWLVSRMQR gi|15668814|ref|NP_247617.1| DADIKFKEMENTKKVLERLYLFAKEIAERRGETGEEFSYIDKWLLSRLYK gi|14590812|ref|NP_142884.1| DFDWRRKEVGKLRKQIERFYELISQFAEYEVKGNVELKDIDRWMLHRLNK gi|14521079|ref|NP_126554.1| DFDWRRKEVGKLRRQLERFYELISQFAEYEAKENVELKTIDKWLLHRLNK gi|14601141|ref|NP_147669.1| DANFEPSVADSAVSLLSQWLDFVRENYGR--PARREERWVDRWFESRLNS * : . : : : : * :*:*: *: gi|11499997|ref|NP_071243.1| AIKEVREAMDNLQTRRAVNAAFFELMNDVRWYLRRG-----GEN---LAI gi|15668814|ref|NP_247617.1| AVKQYDEYMENFELRKAG-ILLYQLLDDLKWYRRRG-----GNNIRVLEE gi|14590812|ref|NP_142884.1| AIKETTNALEEFRTRTAVQWAFYSIMNDLRWYLRRTEGRDDEAKRYVLRT gi|14521079|ref|NP_126554.1| AIEGTTKALEEFRTRTAVQWAFYSIMNDLRWYMRRTEGRDDEAKRFVLRK gi|14601141|ref|NP_147669.1| TIARVTRLMEEANFKTALVEAWYKLQESYRWYLRRSGG---EPREDLLRR :: . ::: . : * :.: :. :** ** . * gi|11499997|ref|NP_071243.1| ILDDWIKLLAPFAPHICEELWHLK-HDSYVSLESYPEYDETRVDEEAERI gi|15668814|ref|NP_247617.1| FLEVIIKLMMPFTPHLCEEMWEILGKEGFVSLAKFPEVKEEFINDEIEKG gi|14590812|ref|NP_142884.1| LADVWVRLMAPFTPHICEELWEKLGGEGFVSLAKWPEPVEEWWNETIEAE gi|14521079|ref|NP_126554.1| LADIWVRLMAPFTPHICEELWEKLGGEGFVSLAKWPEPVDEWWNEEVEVE gi|14601141|ref|NP_147669.1| FIEVQTLLIAPFAPHTAEEAWEAMGREGFASTASWPEPDESKISPEVEAA : : *: **:** .** *. :.:.* .:** : . * gi|11499997|ref|NP_071243.1| EEYLRNLVEDIQEIKKFVS--DAKEVYIAPAEDWKVKAAKVVAESG---- gi|15668814|ref|NP_247617.1| EEYLKAVMEDIKEIINVAKV-QPKRIYLYTADDWKYEILKIIKENEGK-- gi|14590812|ref|NP_142884.1| EEFIRSVMEDIKEIIEVAKIENAKRAYIYTAEDWKWKVAEVVSEKR---- gi|14521079|ref|NP_126554.1| EDFIKSLIEDIKEIIEVAKIESPKRAYIYTAPEWKWKVYEVVAEKR---- gi|14601141|ref|NP_147669.1| EETVQAVLEDAREVLSLIG--GADTLVVTVAAEWKYRAVEAVRRARERGA *: :: ::** :*: .. .. : * :** . : : . gi|11499997|ref|NP_071243.1| DVGEAMKQLMQDEELRKLGKEVSNFVKKIFKD----RKKLMLVKEWEVLQ gi|15668814|ref|NP_247617.1| TIKELMPIIMKNPEFRKYGKEIPKLVNQLIK------LNAEIINEVEVLE gi|14590812|ref|NP_142884.1| DFKSSMEELMKDSEIRKHGKEVAKIVQKLIKE---RTFDVKRINEEKALR gi|14521079|ref|NP_126554.1| EFKSAMAELMKDEEIRKHGKEVAKLVQAIIKE---RAFDVKRIDEEKVLR gi|14601141|ref|NP_147669.1| SMKEALREAFKVEGVDKR--EAARLVQQLSRAPEVLRRAAPRSVELEALR . . : :: . * * ..:*: : : * :.*. gi|11499997|ref|NP_071243.1| QNLKFIENETGLKVILDTQRVPEEKRRQ-AVPGKPAIYVA---- gi|15668814|ref|NP_247617.1| NAKEFLKKEVGVEDIIINGEDKANKKRV-AIPFKPAIYLE---- gi|14590812|ref|NP_142884.1| EAKEFMEKELGIEIIINPTEDKGGKKKQ-AMPLKPAIFIE---- gi|14521079|ref|NP_126554.1| ESKDFLEKELGLEVIINPEEDKGGKKRQ-AIPLKPAVFIE---- gi|14601141|ref|NP_147669.1| DAAQLLQEELGVKVVVETEEDGGSPRRANALPGRPALYAEKRGG : .::::* *:: :: . :: *:* :**::Tree construction
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|11499997|ref|NP_071243.1| 994 aa Sequence 2: gi|15668814|ref|NP_247617.1| 994 aa Sequence 3: gi|14590812|ref|NP_142884.1| 994 aa Sequence 4: gi|14521079|ref|NP_126554.1| 994 aa Sequence 5: gi|14601141|ref|NP_147669.1| 994 aa Phylogenetic tree file created: [clustal.ph]+---------------------------1:gi|11499997|ref|NP_071243.1| | --6 +-------------------------2:gi|15668814|ref|NP_247617.1| | +--7 | | | +---3:gi|14590812|ref|NP_142884.1| +--9 +--------------------8 | +---4:gi|14521079|ref|NP_126554.1| | +-----------------------------------5:gi|14601141|ref|NP_147669.1|Midpoint-rooted tree:
+---------------------------------------1:gi|11499997|ref|NP_071243.1| +-----9 | | +-------------------------------------2:gi|15668814|ref|NP_247617.1| | +--7 --6 | +------3:gi|14590812|ref|NP_142884.1| | +-----------------------------8 | +------4:gi|14521079|ref|NP_126554.1| | +----------------------------------------------5:gi|14601141|ref|NP_147669.1|Remember, this is an unrooted tree!
Bootstrapping tree
CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Clustal Sequence 1: gi|11499997|ref|NP_071243.1| 994 aa Sequence 2: gi|15668814|ref|NP_247617.1| 994 aa Sequence 3: gi|14590812|ref|NP_142884.1| 994 aa Sequence 4: gi|14521079|ref|NP_126554.1| 994 aa Sequence 5: gi|14601141|ref|NP_147669.1| 994 aa Phylogenetic tree file created: [clustal.ph]