Title | Comparison of endogenous and overexpressed MyoD shows enhanced binding of physiologically bound sites. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Yao Z, Fong AP, Cao Y, Ruzzo WL, Gentleman RC, Tapscott SJ |
Journal | Skeletal Muscle |
Volume | 3 |
Issue | 1 |
Pagination | 8 |
Date or Month Published | April 8 |
ISSN | 2044-5040 |
Abstract | BACKGROUND: Transcription factor overexpression is common in biological experiments and transcription factor amplification is associated with many cancers, yet few studies have directly compared the DNA-binding profiles of endogenous versus overexpressed transcription factors. METHODS: We analyzed MyoD ChIP-seq data from C2C12 mouse myotubes, primary mouse myotubes, and mouse fibroblasts differentiated into muscle cells by overexpression of MyoD and compared the genome-wide binding profiles and binding site characteristics of endogenous and overexpressed MyoD. RESULTS: Overexpressed MyoD bound to the same sites occupied by endogenous MyoD and possessed the same E-box sequence preference and co-factor site enrichments, and did not bind to new sites with distinct characteristics. CONCLUSIONS: Our data demonstrate a robust fidelity of transcription factor binding sites over a range of expression levels and that increased amounts of transcription factor increase the binding at physiologically bound sites. |
DOI | 10.1186/2044-5040-3-8 |
Downloads | http://www.ncbi.nlm.nih.gov/pubmed/23566431?dopt=Abstract |
Alternate Journal | Skelet Muscle |
Citation Key | 9215 |
PubMed ID | 23566431 |