|Title||Atherosclerotic plaque smooth muscle cells have a distinct phenotype. |
|Publication Type||Journal Article |
|Year of Publication||2004 |
|Authors||Mulvihill ER, Jaeger J, Sengupta R, Ruzzo WL, Reimer C, Lukito S, Schwartz SM |
|Journal||Arteriosclerosis, thrombosis, and vascular biology |
|Date or Month Published||2004 Jul |
|Keywords||Antigens, CD95, Apoptosis, Arteriosclerosis, Cell Division, Cells, Cultured, Enzyme Induction, Extracellular Matrix Proteins, Fas Ligand Protein, Gene Expression Profiling, Glutathione Transferase, Humans, Membrane Glycoproteins, Muscle Proteins, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Oligonucleotide Array Sequence Analysis, Phenotype, Polymerase Chain Reaction, Tunica Media |
|Abstract||OBJECTIVE: The present study addresses the question, "Are plaque smooth muscles cells (SMCs) genetically distinct from medial SMCs as reflected by the ability to maintain a distinctive expression phenotype in vitro?"
METHODS AND RESULTS: Multiple cell strains were developed from carotid endarcterectomy specimens, and quadruplicate array hybridizations were completed for each sample. A new normalization protocol was developed and used to analyze the data. Permutation analysis suggests that most of the significant differences in expression could not have occurred by chance. A broad pattern of significant expression differences, consisting of almost 5% of the genes probed, was detected. Quantitative polymerase chain reaction (QPCR) confirmation was found in 70% of a subset of genes selected for validation.
CONCLUSIONS: The SMC cultures were nearly indistinguishable by morphological features, population doubling time, and sensitivity to cell death induced by Fas cross-linking. Surprisingly, array expression analysis identified differences so extensive that we conclude that plaque and medial SMCs are distinctly different SMC cell types. |
|Alternate Journal||Arterioscler. Thromb. Vasc. Biol. |
|Citation Key||1885 |
|PubMed ID||15142862 |