TitleA glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Publication TypeJournal Article
Year of Publication2004
AuthorsMandal M, Lee M, Barrick JE, Weinberg Z, Emilsson G M, Ruzzo WL, Breaker RR
JournalScience (New York, N.Y.)
Date or Month Published2004 Oct 8
Keywords5' Untranslated Regions, Allosteric Regulation, Allosteric Site, Bacillus subtilis, Base Pairing, Base Sequence, Binding Sites, Gene Expression Regulation, Bacterial, Glycine, Ligands, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Operon, RNA, Bacterial, RNA, Messenger, Transcription, Genetic, Vibrio cholerae
AbstractWe identified a previously unknown riboswitch class in bacteria that is selectively triggered by glycine. A representative of these glycine-sensing RNAs from Bacillus subtilis operates as a rare genetic on switch for the gcvT operon, which codes for proteins that form the glycine cleavage system. Most glycine riboswitches integrate two ligand-binding domains that function cooperatively to more closely approximate a two-state genetic switch. This advanced form of riboswitch may have evolved to ensure that excess glycine is efficiently used to provide carbon flux through the citric acid cycle and maintain adequate amounts of the amino acid for protein synthesis. Thus, riboswitches perform key regulatory roles and exhibit complex performance characteristics that previously had been observed only with protein factors.
Alternate JournalScience
Citation Key1884
PubMed ID15472076